Delfini C, Grilli G, Polchi P, Porcellini A, Lucarelli G
Ric Clin Lab. 1979 Jan-Mar;9(1):61-6. doi: 10.1007/BF02905028.
Normal and pathological lymphoid cells, collected from different sources, were cryopreserved using a programmed freezing procedure. With this cryopreservation technique, the percentage of T and B cell surface markers and the proliferative response to mitogens were not influenced by 21 and 35 days of storage in liquid nitrogen. The recovery percentage of the lymphoid cells was satisfactory when fetal calf serum was added, as a protein source, to the medium during freezing and thawing phases, while a very low percentage of cells was recovered if the fetal calf serum was omitted.
从不同来源收集的正常和病理性淋巴细胞，采用程序冷冻法进行冷冻保存。采用这种冷冻保存技术，T细胞和B细胞表面标志物的百分比以及对有丝分裂原的增殖反应不受在液氮中保存21天和35天的影响。在冷冻和解冻阶段，当向培养基中添加胎牛血清作为蛋白质来源时，淋巴细胞的回收率令人满意，而如果省略胎牛血清，则细胞回收率非常低。
