Department of Biomedical Engineering, Dongguk University, Seoul 04620, Korea.
Department of Life Science, Dongguk University, Seoul 04620, Korea.
Sensors (Basel). 2019 Sep 10;19(18):3893. doi: 10.3390/s19183893.
Food intolerance is delayed adverse food reactions which follow consumption of specific foods. The underlying mechanisms are not well understood, but food intolerance is often considered as a type 2 hypersensitivity reaction mediated by immunoglobulin G (IgG) antibody. To understand the causes of food intolerance, it is important to investigate sensitization patterns of food-specific IgGs (sIgG) in relation to dietary patterns and physical conditions. Conventional approaches to measure serological IgGs often require large volumes of serum, thus are not suitable for highly multiplexed assays. To overcome this impracticality, we developed a highly sensitive method to screen the sIgGs and other antibody isotypes against 66 antigens with minimal amount of serums. We prepared a microarray by immobilizing food antigens on activated glass slides. Human sera and their dietary information were obtained from 30 subjects. Aliquots (200 nl) of sera were analyzed against 66 food antigens in parallel. sIgG levels were determined and analyzed in relation to subjects' dietary patterns. The levels of antibody isotypes were also examined to understand the relationship between allergy and food intolerance. The developed microarray showed exceptional performances in antibody screening and demonstrated the potential to be used as an automated assay system.
食物不耐受是指食用特定食物后出现的延迟性不良反应。其潜在机制尚未完全阐明，但食物不耐受通常被认为是由免疫球蛋白 G（IgG）抗体介导的 2 型超敏反应。为了了解食物不耐受的原因，重要的是要研究与饮食模式和身体状况相关的食物特异性 IgGs（sIgG）的致敏模式。传统的血清学 IgG 检测方法通常需要大量的血清，因此不适合高通量检测。为了克服这一不切实际的情况，我们开发了一种高度敏感的方法，使用最小量的血清来筛选针对 66 种抗原的 sIgG 和其他抗体同种型。我们将食物抗原固定在活化的玻片上，制备了微阵列。从 30 名受试者中获得了人血清及其饮食信息。将 200 nl 等分试样的血清与 66 种食物抗原平行分析。测定并分析了 sIgG 水平与受试者饮食模式的关系。还检查了抗体同种型的水平，以了解过敏与食物不耐受之间的关系。所开发的微阵列在抗体筛选方面表现出色，并显示出作为自动化检测系统的潜力。
