Volpi Nicola, Maccari Francesca
Department of Biologia Animale, Biological Chemistry Section, University of Modena and Reggio Emilia, Modena, Italy.
J Immunoassay Immunochem. 2009;30(1):51-69. doi: 10.1080/15321810802571903.
Using an optimized and validated ELISA method, we performed a serum test for assaying the binding capacity of serum IgG to proteins extracted from approx. 160 different foods to investigate the reactivity of specific IgG antibodies in the Italian population composed of 6,879 subjects (4,551 females and 2,328 males). 44 antigens showed an IgG response greater than 10% and only 14 aliments had an elevated reactivity greater than 20%, in particular, milk, from cow and goat, and several milk derivatives, along with egg albumen and yeasts. The IgG response to the high reactive food antigens depending on the age of the 6880 subjects was also analyzed. We demonstrated a high IgG response in a very large subject group to milk and milk derivatives, and egg albumin antigens, and we conclude that the validated ELISA test may be applied for the serum/plasma IgG antibody level determination as a useful indicator of adverse reactions to food and food hypersensitivity.
我们采用优化并经验证的酶联免疫吸附测定（ELISA）方法，进行了一项血清检测，以测定血清IgG与从约160种不同食物中提取的蛋白质的结合能力，从而研究由6879名受试者（4551名女性和2328名男性）组成的意大利人群中特异性IgG抗体的反应性。44种抗原显示IgG反应大于l0%，只有14种食物的反应性升高超过20%，特别是来自奶牛和山羊的牛奶、几种奶制品、蛋清和酵母。我们还分析了6880名受试者中，对高反应性食物抗原的IgG反应与年龄的关系。我们证实在一个非常大的受试者群体中，对牛奶、奶制品和蛋清抗原有很高的IgG反应，并且我们得出结论，经验证的ELISA检测可用于测定血清/血浆IgG抗体水平，作为食物不良反应和食物过敏的有用指标。
