南非农村地区幼儿肠聚集性大肠杆菌毒力基因的流行情况。
Prevalence of Virulence Genes in Enteroaggregative Isolates from Young Children from Rural South Africa.
机构信息
Department of Microbiology, University of Venda, Thohoyandou, South Africa.
Center for Global Health, University of Virginia, Charlottesville, Virginia.
出版信息
Am J Trop Med Hyg. 2019 Nov;101(5):1027-1033. doi: 10.4269/ajtmh.19-0192.
In this study, we report on the prevalence of 19 virulence genes in enteroaggregative (EAEC) isolates from northern South Africa. Stool samples obtained prospectively from 97 children from 1 to 12 months of age were analyzed, and EAEC isolates were confirmed based on the presence of or genes. We investigated 177 enteroaggregative isolates for the prevalence of virulence genes using multiplex polymerase chain reaction. The chromosomal gene was detected at higher frequency (48.0%) compared with (26.0%). The gene encoding the open reading frame was the most prevalent putative virulence trait detected among the isolates (150/177; 84.7%). None of the genes was statistically associated with diarrhea ( > 0.05). Detection rates were higher during 7-12 month of life with an association observed for the gene and the age group 7-12 months ( = 0.04). Winter was the season with the highest detection rates. Our data reveal a high prevalence of , , and in South African EAEC isolates. Specific genes may provide additional markers for the study of disease associations with age and season of sample collection.
在这项研究中，我们报告了南非北部肠聚集性大肠杆菌（EAEC）分离株中 19 种毒力基因的流行情况。前瞻性分析了 97 名 1 至 12 个月大的儿童的粪便样本，根据 或 基因的存在确认了 EAEC 分离株。我们使用多重聚合酶链反应调查了 177 株肠聚集性大肠杆菌分离株中毒力基因的流行情况。与 （26.0%）相比，染色体基因 的检出率更高（48.0%）。在检测的分离株中，编码开放阅读框 的基因是最常见的假定毒力特征（150/177；84.7%）。没有一个基因与腹泻有统计学关联（>0.05）。在 7-12 个月的生命中检测率更高，与 7-12 个月年龄组的 基因存在关联（=0.04）。冬季是检测率最高的季节。我们的数据显示，南非 EAEC 分离株中 、 和 的检出率很高。特定基因可能为研究与年龄和样本采集季节相关的疾病提供额外的标记。
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