在高血糖条件下，人脐静脉内皮细胞中Drosha、DGCR8、Dicer和Ago-2基因的表达上调。
The expression of Drosha, DGCR8, Dicer and Ago-2 genes are upregulated in human umbilical vein endothelial cells under hyperglycemic condition.
作者信息
Soufi Farhad Ghadiri, Zonouzi Ali Akbar Poursadegh, Eftekhar Ebrahim, Kamali Kamila, Chegeni Sara Aghakhani, Zonouzi Ahmad Poursadegh
机构信息
Molecular Medicine Research Center, Hormozgan Health institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Genetics and Medical Biotechnology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
出版信息
Endocr Regul. 2018 Jul 1;52(3):123-127. doi: 10.2478/enr-2018-0014.
OBJECTIVES
It has been shown that dysregulation of miRNAs expression contributes to the pathogenesis and progression of the diabetes and diabetes-related complications. Drosha, DGCR8, Dicer, and Ago-2 are involved in the miRNA maturation. The aim of the present study was to investigate the mRNA expression levels of these genes in the human umbilical vein endothelial cells (HUVECs) under hyperglycemic condition.
METHODS
HUVECs were cultured in normo-(5 mM) and hyperglycemic (25 mM) conditions for 24 h. As osmotic control, cells were treated with D-mannitol (25 mM, for 24 h). The mRNA expression levels of Drosha, DGCR8, Dicer and Ago-2 were evaluated using quantitative real-time PCR.
RESULTS
The expression level of Drosha, DGCR8, Dicer, and Ago-2 were increased in hyperglycemic HUVECs compared to the control group.
CONCLUSION
Our results show that under hyperglycemic condition, expression of genes involved in the miRNA maturation was significantly increased in HUVECs. Upregulation of these genes may have role in diabetic complications through the dysregulation of the miRNA expression.
目的
已有研究表明，miRNAs表达失调会导致糖尿病及糖尿病相关并发症的发病机制和病情进展。Drosha、DGCR8、Dicer和Ago-2参与miRNA的成熟过程。本研究旨在探讨高糖条件下人脐静脉内皮细胞（HUVECs）中这些基因的mRNA表达水平。
方法
将HUVECs分别在正常血糖（5 mM）和高血糖（25 mM）条件下培养24小时。作为渗透对照，细胞用D-甘露醇（25 mM，处理24小时）处理。使用定量实时PCR评估Drosha、DGCR8、Dicer和Ago-2的mRNA表达水平。
结果
与对照组相比，高糖处理的HUVECs中Drosha、DGCR8、Dicer和Ago-2的表达水平升高。
结论
我们的结果表明，在高糖条件下，HUVECs中参与miRNA成熟的基因表达显著增加。这些基因的上调可能通过miRNA表达失调在糖尿病并发症中发挥作用。
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