Drosha、22q11.2微缺失综合征关键区域基因8（DGCR8）和Dicer mRNA的过表达在银屑病发病机制中的作用

Overexpression of Drosha, DiGeorge syndrome critical region gene 8 (DGCR8), and Dicer mRNAs in the pathogenesis of psoriasis.

作者信息

Rostami Mogaddam Majid, Safavi Ardabili Nastaran, Shafaeei Yousef, Maleki Nasrollah, Jafari Naser, Jafari Alireza

机构信息

Department of Dermatology, Imam Khomeini Hospital, Ardebil University of Medical Sciences, Ardabil, Iran.

Department of Midwifery, Ardebil Branch, Islamic Azad University, Ardebil, Iran.

出版信息

J Cosmet Dermatol. 2017 Dec;16(4):e48-e53. doi: 10.1111/jocd.12336. Epub 2017 Mar 25.

DOI:10.1111/jocd.12336

PMID:28342199

Abstract

INTRODUCTION

Psoriasis is a complex autoimmune inflammatory disease that occurs in genetically susceptible individuals and presents with the development of inflammatory plaques on the skin. Recent studies have indicated that microRNAs (miRNAs) play important roles in psoriasis.

OBJECTIVE

To investigate whether expression of Drosha, DGCR8, and Dicer mRNAs is involved in the pathogenesis of psoriasis.

METHODS

Biopsies were obtained from involved psoriatic skin (PP), noninvolved psoriatic skin (PN), and healthy skin (NN). Expression of Drosha, Dicer, and DGCR8 was assessed with real-time quantitative real-time PCR in 25 patients with psoriasis and 25 healthy volunteers.

RESULTS

We observed that expression levels of Drosha, Dicer, and DGCR8 were upregulated in patients with psoriasis compared to the control group. However, the Drosha and Dicer expression levels were higher in PP tissues and PN tissues compared to NN tissues, but they were more upregulated in PP tissues compared to PN tissues (P<.001). Although the DGCR8 expression was higher in PP tissues and PN tissues compared to NN tissues, it was more upregulated in PN tissues compared to PP tissues (P<.001).

CONCLUSION

Our data demonstrate that upregulated expression of Drosha, DGCR8, and Dicer mRNAs may be involved in the pathogenesis of psoriasis.

摘要

引言

银屑病是一种复杂的自身免疫性炎症性疾病，发生于遗传易感个体，表现为皮肤上出现炎症性斑块。最近的研究表明，微小RNA（miRNA）在银屑病中起重要作用。

目的

研究Drosha、DGCR8和Dicer mRNA的表达是否参与银屑病的发病机制。

方法

从银屑病受累皮肤（PP）、非受累银屑病皮肤（PN）和健康皮肤（NN）获取活检组织。采用实时定量PCR评估25例银屑病患者和25名健康志愿者中Drosha、Dicer和DGCR8的表达。

结果

我们观察到，与对照组相比，银屑病患者中Drosha、Dicer和DGCR8的表达水平上调。然而，与NN组织相比，PP组织和PN组织中Drosha和Dicer的表达水平更高，但与PN组织相比，PP组织中的上调更为明显（P<0.001）。虽然与NN组织相比，PP组织和PN组织中DGCR8的表达更高，但与PP组织相比，PN组织中的上调更为明显（P<0.001）。