Systems Neuroscience Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Department of Psychiatry, Columbia University Medical Center, 1051 Riverside Drive, New York, NY 10032, USA; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
Neuron. 2019 Nov 6;104(3):601-610.e4. doi: 10.1016/j.neuron.2019.08.006. Epub 2019 Sep 11.
Long-range synchronization of neural oscillations correlates with distinct behaviors, yet its causal role remains unproven. In mice, tests of avoidance behavior evoke increases in theta-frequency (∼8 Hz) oscillatory synchrony between the ventral hippocampus (vHPC) and medial prefrontal cortex (mPFC). To test the causal role of this synchrony, we dynamically modulated vHPC-mPFC terminal activity using optogenetic stimulation. Oscillatory stimulation at 8 Hz maximally increased avoidance behavior compared to 2, 4, and 20 Hz. Moreover, avoidance behavior was selectively increased when 8-Hz stimulation was delivered in an oscillatory, but not pulsatile, manner. Furthermore, 8-Hz oscillatory stimulation enhanced vHPC-mPFC neurotransmission and entrained neural activity in the vHPC-mPFC network, resulting in increased synchrony between vHPC theta activity and mPFC spiking. These data suggest a privileged role for vHPC-mPFC theta-frequency communication in generating avoidance behavior and provide direct evidence that synchronized oscillations play a role in facilitating neural transmission and behavior.
长程神经振荡同步与不同的行为相关，但因果关系仍未得到证实。在小鼠中，回避行为测试会引起腹侧海马体（vHPC）和内侧前额叶皮层（mPFC）之间的θ频（∼8 Hz）振荡同步增加。为了测试这种同步的因果关系，我们使用光遗传学刺激来动态调节 vHPC-mPFC 末梢活动。与 2、4 和 20 Hz 相比，8 Hz 的振荡刺激最大程度地增加了回避行为。此外，当以振荡而非脉冲方式传递 8 Hz 刺激时，回避行为会被选择性地增强。此外，8 Hz 的振荡刺激增强了 vHPC-mPFC 神经传递，并使 vHPC-mPFC 网络中的神经活动同步，从而增加了 vHPCθ 活动和 mPFC 放电之间的同步性。这些数据表明 vHPC-mPFCθ 频率通信在产生回避行为方面具有特权作用，并提供了直接证据表明同步振荡在促进神经传递和行为方面发挥作用。
