Department of Infectious Disease, The First Hospital of Jilin University, Changchun, 130061, China.
Department of Translational Medicine, The First Hospital of Jilin University, Changchun, 130061, China.
Int J Infect Dis. 2019 Dec;89:66-71. doi: 10.1016/j.ijid.2019.09.013. Epub 2019 Sep 12.
To determine blood Brucella DNA loads between brucellosis patients and those without brucellosis.
The patient group included 350 brucellosis patients. The control was composed of 200 subjects without brucellosis. The extracted DNA from blood was tested by quantitative polymerase chain reaction (qPCR). The cutoff value was determined by receiver operating characteristic curve analysis. A portion of the brucellosis patients were monitored by qPCR during therapy.
The detection limit of qPCR was between 1E+01cfu/μL and 1E+08cfu/μL. The standard curve R reached 0.998. The cutoff value was 4E+01cfu/μL, which was determined by comparison of the patient group and the control. The qPCR assay had a specificity of 100% and a sensitivity of 93.14%. The monitoring results showed that the Brucella DNA load decreased in most patients during the first 4 weeks of treatment. One patient with bad treatment compliance showed a rebound.
The qPCR results were in accordance with the course of brucellosis in the clinic. The DNA load often reflects the situation of the Brucella-infected patient. The cutoff value provides an important reference of infection. This qPCR-based method can be used to assist in the diagnosis of brucellosis and to adjust the therapy.
确定布鲁氏菌病患者与非布鲁氏菌病患者之间的血液布鲁氏菌 DNA 载量。
患者组包括 350 例布鲁氏菌病患者，对照组由 200 例无布鲁氏菌病的患者组成。从血液中提取的 DNA 通过实时聚合酶链反应（qPCR）进行检测。通过受试者工作特征曲线分析确定截断值。部分布鲁氏菌病患者在治疗过程中通过 qPCR 进行监测。
qPCR 的检测下限为 1E+01cfu/μL 至 1E+08cfu/μL。标准曲线 R 达到 0.998。通过比较患者组和对照组，确定截断值为 4E+01cfu/μL。qPCR 检测的特异性为 100%，灵敏度为 93.14%。监测结果显示，大多数患者在治疗的前 4 周内布鲁氏菌 DNA 载量下降。一位治疗依从性差的患者出现反弹。
qPCR 结果与临床布鲁氏菌病的病程相符。DNA 载量常反映布鲁氏菌感染患者的情况。截断值为感染提供了重要的参考。这种基于 qPCR 的方法可用于辅助布鲁氏菌病的诊断，并调整治疗方案。
