一种优化和指导 Dsz 酶在体内外 4S 脱硫途径中剂量比的动力学模型。
A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo.
机构信息
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
出版信息
Biotechnol Lett. 2019 Nov;41(11):1333-1341. doi: 10.1007/s10529-019-02730-1. Epub 2019 Sep 14.
OBJECTIVE
To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes.
RESULTS
This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be A:B:C = 1:2:4 and 1:4:2. When BCAD+1A+4B+2C, the conversion rate of dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP) was close to 100% in vitro. When the gene dose of dszC was increased, the HBP yield of the recombinant strain BL21(DE3)/BCAD + C reached approximately 0.012 mM in vivo, which was approximately 6-fold higher than that of the BCAD strain.
CONCLUSIONS
According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency.
目的
利用动力学模型提高生物脱硫率，指导 Dsz 酶的比例。
结果
本研究建立了一个动力学模型，预测 4S 生物脱硫系统中 Dsz 酶的最佳比例为 A：B：C=1：2：4 和 1：4：2。当 BCAD+1A+4B+2C 时，二苯并噻吩（DBT）向 2-羟基联苯（HBP）的转化率接近体外 100%。当 dszC 的基因剂量增加时，重组菌株 BL21（DE3）/BCAD+C 的 HBP 产量在体内达到约 0.012mM，比 BCAD 菌株高约 6 倍。
结论
根据酶动力学模型的预测结果，在脱硫系统中保持较高浓度的 DszC 和 DszB 可以有效提高脱硫效率。
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