Krusius T, Finne J, Andersson L C, Gahmberg C G
Biochem J. 1979 Aug 1;181(2):451-6. doi: 10.1042/bj1810451.
Carbohydrate units of cell-surface glycoproteins of mouse B- and T-lymphocytes, labelled in their sialic acid residues by the periodate/NaB3H4 method and in their galactose residues by the galactose oxidase/NaB3H4 method after neuraminidase treatment, have been studied. Glycopeptides were prepared from the labelled cells by Pronase digestion and fractionated by concanavalin A affinity chromatography into two fractions (A and B). Alkali-labile oligosaccharides were isolated after mild NaOH/NaBH4 treatment by gel filtration. The alkali-labile oligosaccharides were further analysed by t.l.c. To study the relative proportion of neutral mannose-rich carbohydrate units (fraction C) in lymphocyte glycoproteins, glycopeptides were also prepared from unlabelled cells and subjected to concanavalin A affinity chromatography after N-[3H]acetylation of their peptide moiety. The major alkali-labile oligosaccharide component of both cell types was identified as galactosyl-(beta 1 leads to 3)-N-acetylgalactosaminitol. T-Lymphocytes were characterized by a high proportion of this oligosaccharide and a lower proportion of alkali-stable fraction A glycopeptides, whereas the opposite was observed for B-lymphocytes. The relative proportions of the concanavalin A-binding fractions B and C were similar in both cell types. The differences observed may correlate with the different surface properties of B- and T-lymphocytes.
已对小鼠B淋巴细胞和T淋巴细胞细胞表面糖蛋白的碳水化合物单元进行了研究。这些碳水化合物单元通过高碘酸盐/NaB3H4法标记其唾液酸残基，并在神经氨酸酶处理后通过半乳糖氧化酶/NaB3H4法标记其半乳糖残基。通过链霉蛋白酶消化从标记细胞中制备糖肽，并通过伴刀豆球蛋白A亲和色谱法将其分离为两个部分（A和B）。通过凝胶过滤在温和的NaOH/NaBH4处理后分离出碱不稳定寡糖。通过薄层层析对碱不稳定寡糖进行进一步分析。为了研究淋巴细胞糖蛋白中富含中性甘露糖的碳水化合物单元（部分C）的相对比例，还从未标记细胞中制备糖肽，并在其肽部分进行N-[3H]乙酰化后进行伴刀豆球蛋白A亲和色谱分析。两种细胞类型的主要碱不稳定寡糖成分均被鉴定为半乳糖基-（β1→3）-N-乙酰半乳糖胺醇。T淋巴细胞的特征是这种寡糖的比例较高，而碱稳定的A部分糖肽的比例较低，而B淋巴细胞则观察到相反的情况。两种细胞类型中伴刀豆球蛋白A结合部分B和C的相对比例相似。观察到的差异可能与B淋巴细胞和T淋巴细胞的不同表面特性相关。
