Passani S L, Angelini G D, Breckenridge I M, Newby A C
Cardiac Surgery Department, University Hospital of Wales, Cardiff, UK.
Eur J Cardiothorac Surg. 1988;2(4):233-6. doi: 10.1016/1010-7940(88)90077-2.
Endothelial functional integrity was quantified in human saphenous vein by measurement of stimulated (vortex-mixing) rates of prostacyclin production. Prostacyclin production was not inhibited by dimethylsulphoxide (DMSO), glycerol or sucrose at concentrations normally used for cryopreservation. Rapid freezing followed by storage for 3-4 weeks and thawing of veins pretreated with 15% (v/v) dimethylsulphoxide significantly impaired prostacyclin production. In contrast, slow freezing and thawing in the presence, but not the absence, of DMSO led to complete retention of prostacyclin production. We conclude that endothelial function can be preserved during cryostorage.
通过测量前列环素生成的刺激（涡旋混合）速率来量化人隐静脉中的内皮功能完整性。在通常用于冷冻保存的浓度下，前列环素的生成不受二甲基亚砜（DMSO）、甘油或蔗糖的抑制。快速冷冻后储存3 - 4周，并用15%（v/v）二甲基亚砜预处理的静脉解冻后，前列环素的生成显著受损。相比之下，在有DMSO存在（而非不存在）的情况下缓慢冷冻和解冻可使前列环素的生成完全保留。我们得出结论，在冷冻保存期间内皮功能可以得到保留。
