Universität Heidelberg, Medizinische Fakultät Mannheim, Mannheim, Germany.
KIT Campus Nord, Institut für Toxikologie und Genetik, Karlsruhe, Germany.
Lab Anim. 2020 Aug;54(4):391-396. doi: 10.1177/0023677219873684. Epub 2019 Sep 16.
The parenteral administration of hydrophobic substances in vivo requires the use of organic solvents to ensure sufficient solubility and avoid precipitation. Dimethyl sulfoxide is commonly used for this purpose. Based on the common assumption that polyethylene glycol (PEG) is non-toxic, our local regulatory authorities recently recommended the use of PEG instead. However, mice injected intraperitoneally (i.p.) with PEG 200 at a dose of 8 mL/kg (i.e. 9 g/kg) did not tolerate PEG 200 well, and half of the animals had to be euthanized. Our results demonstrate that although PEG 200 is generally considered to be harmless, it can be toxic when injected i.p. and is painful for the recipient mice. Nevertheless, it can be used as a solvent for repeated i.p. injections in mice at a dose of 2 mL/kg (i.e. 2.25 g/kg) without obvious signs of systemic toxicity.
在体内将疏水性物质进行肠胃外给药时，需要使用有机溶剂以确保足够的溶解度并避免沉淀。二甲基亚砜常用于此目的。基于聚乙二醇（PEG）无毒的常见假设，我们当地的监管机构最近建议使用 PEG 代替它。然而，以 8 mL/kg（即 9 g/kg）的剂量经腹腔内（i.p.）注射 PEG 200 的小鼠不能很好地耐受 PEG 200，一半的动物必须被安乐死。我们的结果表明，尽管 PEG 200 通常被认为是无害的，但当经腹腔内注射时它可能有毒，并且对接受注射的小鼠是痛苦的。然而，它可以用作溶剂，以 2 mL/kg（即 2.25 g/kg）的剂量在小鼠中进行重复 i.p. 注射，而没有明显的全身毒性迹象。
