Aoyama Shinya, Hirooka Rina, Shimoda Takeru, Shibata Shigenobu
Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Organization for University Research Initiatives, Waseda University, Tokyo, Japan.
Biochem Biophys Rep. 2019 Sep 10;20:100686. doi: 10.1016/j.bbrep.2019.100686. eCollection 2019 Dec.
Dietary protein intake is important for skeletal muscle protein synthesis. In this study, we investigated the differential effect of protein sources on hypertrophy of plantaris muscle induced by surgical ablation of gastrocnemius and soleus muscles. Six-week old mice were fed diets containing caseinate, whey, or soy as protein sources for 2 weeks. Plantaris muscle hypertrophy was induced by a unilateral ablation of synergistic muscles after a week. Food intake of soy protein-fed mice was higher than that of caseinate and whey-fed mice, resulting in higher body and fat weights. Plantaris muscle weight in sham-operated mice was not different across the groups. Overload-operated plantaris muscle weight and increased ratio of overloaded muscle to sham-operated muscle weights were higher in caseinate-fed mice than in whey- and soy protein-fed mice, suggesting caseinate as a promising protein source for muscle hypertrophy.
膳食蛋白质摄入对于骨骼肌蛋白质合成很重要。在本研究中，我们调查了蛋白质来源对因腓肠肌和比目鱼肌手术切除诱导的跖肌肥大的不同影响。六周龄小鼠被喂食以酪蛋白酸盐、乳清或大豆作为蛋白质来源的饮食，持续2周。一周后通过单侧切除协同肌诱导跖肌肥大。喂食大豆蛋白的小鼠的食物摄入量高于喂食酪蛋白酸盐和乳清的小鼠，导致更高的体重和脂肪量。假手术小鼠的跖肌重量在各组之间没有差异。喂食酪蛋白酸盐的小鼠中，超负荷手术的跖肌重量以及超负荷肌肉与假手术肌肉重量的增加比例高于喂食乳清和大豆蛋白的小鼠，这表明酪蛋白酸盐是一种有前景的促进肌肉肥大的蛋白质来源。
