Faculty of Veterinary Medicine, Department of Biochemistry, Mustafa Kemal University, 31060, Hatay, Turkey.
Faculty of Veterinary Medicine, Department of Biochemistry, Mehmet Akif Ersoy University, 15030, Burdur, Turkey.
Biol Trace Elem Res. 2020 Jul;196(1):131-144. doi: 10.1007/s12011-019-01897-y. Epub 2019 Sep 16.
Tumor microenvironment, genetic, and non-genetic factors are responsible for the atypical metabolic feature of cancer cells. Aberrant activity of PI3K/Akt pathway, increased glycolytic flux, and decreased intracellular pH gradient are the leading causes of this feature. Calcium Fructoborate (CaFB), a sugar-borate ester, has major benefits for human health. The aim of this study was to explore the implication of CaFB on experimentally induced skin cancer in vivo. According to the treatment, 92 female Balb-c mice are divided into six groups: control, CaFB (3 mg/kg/day), 7,12-Dimethylbenz(a)anthracene (DMBA)+12-O-tetradecanoylphorbol-13-acetate (TPA) (97.5 nmol DMBA, 6.5 nmol TPA), T1: CaFB+DMBA+TPA (3 mg/kg/day CaFB together with DMBA), T2: DMBA+CaFB+TPA (3 mg/kg/day CaFB together with TPA), T3: DMBA+TPA+CaFB (3 mg/kg/day CaFB after tumor formation). Topical DMBA and TPA application resulted in a significant increase in the protein levels, immunoreactivity, and mRNA expression of HRAS, HIF1α, Akt, and PTEN (p < 0.05). Moreover, an increase in the number of TUNEL-positive cells was observed in DMBA-TPA group compared with the control group (p < 0.05). CaFB application reduced the protein levels, immunoreactivity, and mRNA expressions of HRAS, HIF1α, Akt, and PTEN and also decreased the number of TUNEL-positive cells. Recent evidence obtained from our study validated that CaFB treatment may have skin cancer-preventing effect.
肿瘤微环境、遗传和非遗传因素是导致癌细胞非典型代谢特征的原因。PI3K/Akt 通路的异常活性、糖酵解通量的增加和细胞内 pH 梯度的降低是导致这种特征的主要原因。钙果硼酸酯（CaFB）是一种糖硼酸酯，对人类健康有重要的益处。本研究旨在探讨 CaFB 对体内实验性诱导皮肤癌的影响。根据治疗方法，92 只雌性 Balb-c 小鼠被分为六组：对照组、CaFB（3mg/kg/天）、7,12-二甲基苯并（a）蒽（DMBA）+12-O-十四烷酰佛波醇-13-乙酸酯（TPA）（97.5nmol DMBA，6.5nmol TPA）、T1：CaFB+DMBA+TPA（3mg/kg/天 CaFB 与 DMBA 一起）、T2：DMBA+CaFB+TPA（3mg/kg/天 CaFB 与 TPA 一起）、T3：DMBA+TPA+CaFB（3mg/kg/天 CaFB 在肿瘤形成后）。DMBA 和 TPA 的局部应用导致 HRAS、HIF1α、Akt 和 PTEN 的蛋白水平、免疫反应性和 mRNA 表达显著增加（p<0.05）。此外，与对照组相比，DMBA-TPA 组中 TUNEL 阳性细胞的数量增加（p<0.05）。CaFB 应用降低了 HRAS、HIF1α、Akt 和 PTEN 的蛋白水平、免疫反应性和 mRNA 表达，并减少了 TUNEL 阳性细胞的数量。我们的研究最近获得的证据证实，CaFB 治疗可能具有预防皮肤癌的作用。
