Tepedelen Burcu Erbaykent, Korkmaz Mehmet, Tatlisumak Ertugrul, Uluer Elgin Türköz, Ölmez Ercüment, Değerli İsmail, Soya Elif, İnan Sevinç
Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
Department of Anatomy, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
Biol Trace Elem Res. 2017 Aug;178(2):210-217. doi: 10.1007/s12011-016-0918-6. Epub 2016 Dec 28.
Evidences about the preventive and therapeutic effects of boron compounds on cancer have been increasing in the last years. Although calcium fructoborate (CaFB) is used as a nutritional supplement, data about its preventive and therapeutic effects on neoplastic transformations are limited. In the present study, the various concentrations of CaFB were applied to the MDA-MB-231 metastatic breast cancer cell line. First, we examined the cytotoxic effect and IC value of CaFB by MTT assay. For the evaluation of the DNA damage, apoptosis and metastatic potential, expression levels of ATM, pATM, PARP, p53, p-p53, caspase-3, caspase-9, and VEGF were investigated by using immunoblotting and immunohistochemical methods. Cell viability was significantly reduced at 50 μM CaFB treatment. pATM, p-p53, and caspase-9 levels increased significantly in all groups; furthermore, there was approximately 12.5-, 2.4-, and 10.7-fold increase, respectively, for 100 μM CaFB treatment. ATM and p53 levels did not change with CaFB treatment, but PARP levels significantly 2.5-fold decreased. While VEGF immunoreactivity decreased in all groups, significant increase in caspase-3 immunoreactivity was observed only in the group treated with 50 μM CaFB (p < 0,001). Our results imply that CaFB may have therapeutic potential as well as preventive benefits in cancer.
近年来，有关硼化合物对癌症预防和治疗作用的证据不断增加。尽管果糖硼酸钙（CaFB）被用作营养补充剂，但其对肿瘤转化的预防和治疗作用的数据有限。在本研究中，将不同浓度的CaFB应用于MDA-MB-231转移性乳腺癌细胞系。首先，我们通过MTT法检测了CaFB的细胞毒性作用和IC值。为了评估DNA损伤、细胞凋亡和转移潜能，采用免疫印迹和免疫组化方法研究了ATM、pATM、PARP、p53、p-p53、caspase-3、caspase-9和VEGF的表达水平。在50μM CaFB处理时，细胞活力显著降低。所有组中pATM、p-p53和caspase-9水平均显著升高；此外，在100μM CaFB处理时，它们分别增加了约12.5倍、2.4倍和10.7倍。CaFB处理后ATM和p53水平未发生变化，但PARP水平显著降低了2.5倍。虽然所有组中VEGF免疫反应性均降低，但仅在50μM CaFB处理组中观察到caspase-3免疫反应性显著增加（p < 0.001）。我们的结果表明，CaFB在癌症中可能具有治疗潜力以及预防益处。
