突尼斯TLR2基因（-196至-174位插入/缺失）和TLR3基因1377C>T变异与头颈癌之间风险增加。
Increased risks between TLR2 (-196 to -174 ins/del) and TLR3 1377C>T variants and head and neck cancers in Tunisia.
作者信息
Makni Lamia, Zidi Sabrina, Barbiroud Mouadh, Ahmed Amira Ben, Gazouani Ezzedine, Mezlini Amel, Stayoussef Mouna, Yacoubi-Loueslati Besma
机构信息
Laboratory of Mycology, Pathologies, and Biomarkers: LR16ES05, Department of Biology, Faculty of Sciences, El Manar University, Tunis, Tunisia.
Laboratory of Venoms and Therapeutic Molecules, Pasteur Institute of Tunisia, El Manar University, Tunis, Tunisia.
出版信息
Cent Eur J Immunol. 2019;44(2):144-149. doi: 10.5114/ceji.2019.87065. Epub 2019 Jul 30.
INTRODUCTION
Previous studies have highlighted the importance of polymorphisms of toll-like receptors (TLRs) in the pathogenesis of certain cancers, including head and neck cancers (HNC).
AIM OF THE STUDY
The aim of this study was to evaluate the association of TLR2 (-196 to -174 ins/del) and TLR3 (1377 C>T) as potential risk factors for HNC in Tunisians.
MATERIAL AND METHODS
A case-control study including 246 HNC patients (174 nasopharyngeal carcinoma - NPC and 72 laryngeal cancer - LC) and 250 healthy controls. Genotyping was done by using PCR and PCR-RFLP methods.
RESULTS
Higher minor allele frequencies of TLR2 (-196 to -174 ins/del) and TLR3 1377 C>T polymorphisms were seen in HNC, NPC, and LC compared to controls. In addition, higher increased HNC, NPC, and LC risk was associated with TLR2 ins/del and TLR2 del/del genotypes (p < 0.0001). Positive association with HNC, NPC, and LC risk was seen with TLR2 del-containing genotypes (ins/del + del/del) (p < 0.0001). The T/T genotype of TLR3 is associated with HNC, NPC, and LC susceptibility (p < 0.0001). Positive association with HNC and NPC risk was seen with TLR3 T allele carriers (C/T + T/T) (p < 0.0001). Increased frequency of T-ins, C-del, and T-del haplotypes was revealed in HNC and NPC cases than healthy controls; however, T-del was significantly higher in LC cases.
CONCLUSIONS
Our results demonstrate an increased risk of HNC, NPC, and LC with TLR2 ins/del, TLR2 del/del, and TLR3 T/T genotypes. And positive association with T-ins, C-del, and T-del haplotypes with HNC and NPC and T-del haplotype with LC.
引言
先前的研究强调了 Toll 样受体（TLR）多态性在某些癌症（包括头颈癌，HNC）发病机制中的重要性。
研究目的
本研究旨在评估 TLR2（-196 至-174 插入/缺失）和 TLR3（1377 C>T）作为突尼斯人患 HNC 的潜在风险因素之间的关联。
材料与方法
一项病例对照研究，纳入 246 例 HNC 患者（174 例鼻咽癌-NPC 和 72 例喉癌-LC）以及 250 名健康对照。采用聚合酶链反应（PCR）和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因分型。
结果
与对照组相比，在 HNC、NPC 和 LC 中观察到 TLR2（-196 至-174 插入/缺失）和 TLR3 1377 C>T多态性的次要等位基因频率更高。此外，TLR2 插入/缺失和 TLR2 缺失/缺失基因型与 HNC、NPC 和 LC 风险增加相关（p < 0.0001）。含 TLR2 缺失的基因型（插入/缺失+缺失/缺失）与 HNC、NPC 和 LC 风险呈正相关（p < 0.0001）。TLR3 的 T/T 基因型与 HNC、NPC 和 LC 易感性相关（p < 0.0001）。TLR3 T 等位基因携带者（C/T + T/T）与 HNC 和 NPC 风险呈正相关（p < 0.0001）。与健康对照相比，HNC 和 NPC 病例中 T-插入、C-缺失和 T-缺失单倍型的频率增加；然而，T-缺失在 LC 病例中显著更高。
结论
我们的结果表明，TLR2 插入/缺失、TLR2 缺失/缺失和 TLR3 T/T 基因型会增加患 HNC、NPC 和 LC 的风险。并且 T-插入、C-缺失和 T-缺失单倍型与 HNC 和 NPC 呈正相关，T-缺失单倍型与 LC 呈正相关。
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