食欲素A促进绵羊颗粒细胞分泌孕酮。
Orexin A promotes granulosa cell secretion of progesterone in sheep.
作者信息
Li M, Zu N, Zhang C S, Xie M Y, Liu Y Z, Xu X J
机构信息
MSc Student in Developmental Biology, Animal Biotechnology Laboratory, College of Veterinary Medicine, The Inner Mongolia Agricultural University, Hohehot, Inner Mongolia, China.
Agriculture and Animal Husbandry Department of Inner Mongolia Autonomous Region, Hohehot, Inner Mongolia, China.
出版信息
Iran J Vet Res. 2019 Spring;20(2):136-142.
BACKGROUND
Orexin A, a small-molecule peptide, can regulate female hormones, but limited evidence for its mechanism of activity exists in ovine.
AIMS
The objective of this study was to investigate the effect of orexin A on progesterone (P4) secretion in cultured granulosa of sheep follicles.
METHODS
Sheep ovarian granulosa were isolated and identified, pre-incubated with luteinizing hormone (LH) (2.5 IU/ml), follicle-stimulating hormone (FSH) (2.5 IU/ml), or oestrogen (1 µg/ml); and cultured . The pretreated sheep ovarian granulosa were subsequently cultured with different concentrations (1 nM, 10 nM, 58 nM, 100 nM, and 145 nM) of orexin A for varying amounts of time (0 h, 24 h, 48 h, and 72 h). Then, the expression levels of P4, steroidogenic acute regulatory protein (StAR), 3β-Hydroxysteroid dehydrogenase (3β-HSD) and cytochrome P450 (CYP11) were determined.
RESULTS
The results showed that the sheep ovarian granulosa were correctly identified. The different concentrations of orexin A promoted the secretion of P4 from granulosa in the ovine ovary compared with that in the control. The expression of StAR, 3β-HSD and P450 (CYP11) gradually increased, and then decreased with increasing concentrations of orexin A, but the expression of P450 (CYP11) decreased with the increase of time.
CONCLUSION
These results revealed that orexin A promotes the secretion of P4 by regulating the expression of StAR, 3β-HSD, and P450 (CYP11). Understanding the mechanism underlying the promotion of P4 by orexin A could open new therapeutic possibilities in the treatment of hormone homeostasis.
背景
食欲素A是一种小分子肽，可调节女性激素，但在绵羊中关于其活性机制的证据有限。
目的
本研究旨在探讨食欲素A对培养的绵羊卵泡颗粒细胞中孕酮（P4）分泌的影响。
方法
分离并鉴定绵羊卵巢颗粒细胞，用促黄体生成素（LH）（2.5 IU/ml）、促卵泡生成素（FSH）（2.5 IU/ml）或雌激素（1 µg/ml）进行预孵育，然后进行培养。随后，将预处理的绵羊卵巢颗粒细胞与不同浓度（1 nM、10 nM、58 nM、100 nM和145 nM）的食欲素A在不同时间（0小时、24小时、48小时和72小时）进行培养。然后，测定P4、类固醇生成急性调节蛋白（StAR）、3β-羟基类固醇脱氢酶（3β-HSD）和细胞色素P450（CYP11）的表达水平。
结果
结果表明绵羊卵巢颗粒细胞被正确鉴定。与对照组相比，不同浓度的食欲素A促进了绵羊卵巢颗粒细胞中P4的分泌。StAR、3β-HSD和P450（CYP11）的表达随食欲素A浓度增加而逐渐升高，然后降低，但P450（CYP11）的表达随时间增加而降低。
结论
这些结果表明，食欲素A通过调节StAR、3β-HSD和P450（CYP11）的表达促进P4的分泌。了解食欲素A促进P4分泌的机制可能为激素稳态治疗开辟新的治疗途径。
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