Wei S, Gong Z, Guo H, Zhang T, Ma Z
Department of Reproduction Endocrinology, Life Science and Engineering College, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China.
Affiliated Hospital, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China.
Iran J Vet Res. 2017 Spring;18(2):79-85.
The study aimed to investigate the effects of FSH and eCG on the ovarian and follicular development, expression levels of FSHR and caspase-9 of ovaries . One hundred and five prepuberty mice were allocated into FSH-1, FSH-2, FSH-3, eCG-1, eCG-2, eCG-3 groups and control group (CG). Mice in FSH-1, FSH-2 and FSH-3 were intramuscularly injected with 5, 10 and 20 IU FSH twice (on day 0 and 4), respectively. Mice in eCG-1, eCG-2 and eCG-3 were intraperitoneally injected with 10, 20 and 40 IU eCG on day 0 and 4. Mice in the CG were injected with 0.5 ml normal saline on day 0 and 4. Left and right ovaries of each mouse were dissected aseptically on days 7, 14 and 21, respectively. The results showed that on days 14 and 21 the ovarian sizes and follicle numbers of FSH-3 and eCG-3 groups were greater than CG (P<0.05). FSHR mRNA of FSH-2 and eCG-1 were higher than CG on days 14 and 21 (P<0.05). FSHR proteins of FSH-3 were higher than CG on days 14 and 21 (P<0.05). Caspase-9 mRNA in FSH and eCG groups was less than CG. There were positive correlations between follicle numbers and FSH and eCG doses. FSHR protein expressions had positive correlations between ovarian weights and sizes of ovary and follicle numbers (r=0.971, P<0.05) in FSH-treated mice. Serum FSH concentrations of FSH-2, FSH-3, eCG-2 and eCG-3 groups were greater than that of CG. In conclusion, eCG and FSH promoted the ovarian development, follicle genesis, FSH secretion, FSHR mRNA and protein expressions in ovaries of mice. FSH and eCG inhibited the expression of ovarian caspase-9 mRNA.
该研究旨在探讨促卵泡素（FSH）和孕马血清促性腺激素（eCG）对小鼠卵巢及卵泡发育、卵巢中FSH受体（FSHR）和半胱天冬酶-9表达水平的影响。将105只青春期前小鼠分为FSH-1、FSH-2、FSH-3、eCG-1、eCG-2、eCG-3组及对照组（CG）。FSH-1、FSH-2和FSH-3组小鼠分别于第0天和第4天肌肉注射5、10和20国际单位（IU）的FSH，共两次。eCG-1、eCG-2和eCG-3组小鼠于第0天和第4天腹腔注射10、20和40 IU的eCG。CG组小鼠于第0天和第4天注射0.5毫升生理盐水。分别于第7、14和21天无菌解剖每只小鼠的左右卵巢。结果显示，在第14天和第21天，FSH-3组和eCG-3组的卵巢大小和卵泡数量均大于CG组（P<0.05）。在第14天和第21天，FSH-2组和eCG-1组的FSHR mRNA高于CG组（P<0.05）。在第14天和第21天,FSH-3组的FSHR蛋白高于CG组（P<0.05）。FSH组和eCG组中的半胱天冬酶-9 mRNA少于CG组。卵泡数量与FSH和eCG剂量之间存在正相关。在FSH处理的小鼠中，FSHR蛋白表达与卵巢重量、卵巢大小和卵泡数量之间存在正相关（r=0.971，P<0.05）。FSH-2、FSH-3、eCG-2和eCG-3组的血清FSH浓度高于CG组。总之，eCG和FSH促进了小鼠卵巢发育、卵泡发生、FSH分泌、卵巢中FSHR mRNA和蛋白表达。FSH和eCG抑制了卵巢半胱天冬酶-9 mRNA的表达。
