Martínez-Carrillo B E, Rosales-Gómez C A, Ramírez-Durán N, Reséndiz-Albor A A, Escoto-Herrera J A, Mondragón-Velásquez T, Valdés-Ramos R, Castillo-Cardiel A
Laboratorio de Investigación en Nutrición, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan, Esquina Jesús Carranza, s/n, Colonia Moderna de la Cruz, C.P. 50180, Toluca, Mexico.
Laboratorio de Microbiología Médica y Ambiental, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan, Esquina Jesús Carranza, s/n, Colonia Moderna de la Cruz, C.P. 50180, Toluca, Mexico.
Int J Food Sci. 2019 Aug 20;2019:9619020. doi: 10.1155/2019/9619020. eCollection 2019.
The consumption of sweeteners has increased as a measure to reduce the consumption of calories and thus combat obesity and diabetes. Sweeteners are found in a large number of products, so chronic consumption has been little explored. The objective of the study was to evaluate the effect of chronic sweetener consumption on the microbiota and immunity of the small intestine in young mice. We used 72 CD1 mice of 21 days old, divided into 3 groups: (i) No treatment, (ii) Group A (6 weeks of treatment), and (iii) Group B (12 weeks of treatment). Groups A and B were divided into 4 subgroups: Control (CL), Sucrose (Suc), Splenda® (Spl), and Svetia® (Sv). The following were determined: anthropometric parameters, percentage of lymphocytes of Peyer's patches and lamina propria, IL-6, IL-17, leptin, resistin, C-peptide, and TNF-. From feces, the microbiota of the small intestine was identified. The BMI was not modified; the mice preferred the consumption of Splenda® and Svetia®. The percentage of CD3 lymphocytes in Peyer's patches was increased. In the lamina propria, Svetia® increased the percentage of CD3 lymphocytes, but Splenda® decreases it. The Splenda® and Svetia® subgroups elevate leptin, C-peptide, IL-6, and IL-17, with reduction of resistin. The predominant genus in all groups was . The chronic consumption of sweeteners increases the population of lymphocytes in the mucosa of the small intestine. Maybe, Bacillus have the ability to adapt to sweeteners regardless of the origin or nutritional contribution of the same.
作为减少热量摄入从而对抗肥胖和糖尿病的一项措施，甜味剂的消费量有所增加。大量产品中都含有甜味剂，因此对长期食用甜味剂的情况研究较少。本研究的目的是评估长期食用甜味剂对幼鼠小肠微生物群和免疫力的影响。我们使用了72只21日龄的CD1小鼠，分为3组：（i）不治疗组，（ii）A组（治疗6周），和（iii）B组（治疗12周）。A组和B组又分为4个亚组：对照组（CL）、蔗糖组（Suc）、Splenda®组（Spl）和Svetia®组（Sv）。测定了以下指标：人体测量参数、派尔集合淋巴结和固有层淋巴细胞百分比、白细胞介素-6（IL-6）、白细胞介素-17（IL-17）、瘦素、抵抗素、C肽和肿瘤坏死因子-α（TNF-α）。从小鼠粪便中鉴定出小肠微生物群。体重指数未发生改变；小鼠更喜欢食用Splenda®和Svetia®。派尔集合淋巴结中CD3淋巴细胞百分比增加。在固有层中，Svetia®增加了CD3淋巴细胞百分比，但Splenda®降低了该百分比。Splenda®组和Svetia®组亚组升高了瘦素、C肽、IL-6和IL-17，并降低了抵抗素。所有组中占主导地位的菌属是……长期食用甜味剂会增加小肠黏膜中淋巴细胞的数量。也许，芽孢杆菌具有适应甜味剂的能力，而不论甜味剂的来源或营养成分如何。
