School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Biomed Res Int. 2019 Aug 18;2019:5476076. doi: 10.1155/2019/5476076. eCollection 2019.
The blood of cancer patients is in a state of hypercoagulability, easily leading to thrombosis. Anemia is also a complication of tumors. Anemia and thrombosis affect the treatment of tumor patients.
Ginsenosides Rb were extracted from the stems and leaves of American ginseng using water-saturated ethanol and ethyl acetate in silica gel column. Tumor mice model was established by injecting H hepatocellular carcinoma cells into the axilla of mice. Mice were randomly divided into 6 groups: normal control group, model control group, positive control group, low dose group (7 mg/kg), middle dose group (14 mg/kg), and high dose group (35 mg/kg). After 18 days, the blood was obtained by picking the eyeball of mice. The levels of red blood cells (RBC), hemoglobin (HGB), neutrophils/lymphocytes radio (NLR), platelets (PLT), platelet distribution width (PDW), fibrinogen (FIB), and D-Dimer (D-D) were measured and compared in each group of mice.
The content of obtained ginsenosides Rb reached 90.05%. This extraction process was simple and reliable. Middle dose of ginsenosides Rb could significantly increase RBC and HGB levels (P<0.05). Moreover, ginsenosides Rb could significantly reduce NLR, PLT, PDW, FIB, and D-D (P<0.01).
ginsenosides Rb could significantly improve anaemia and hypercoagulation of blood in cancer mice. Ginsenosides Rb are a potential anticoagulant and antianemia drug in treating cancer.
癌症患者的血液处于高凝状态，容易导致血栓形成。贫血也是肿瘤的并发症。贫血和血栓形成会影响肿瘤患者的治疗。
采用水饱和乙醇和硅胶柱中的乙酸乙酯从西洋参的茎和叶中提取人参皂苷 Rb。通过向小鼠的腋窝注射 H 肝癌细胞建立肿瘤小鼠模型。将小鼠随机分为 6 组：正常对照组、模型对照组、阳性对照组、低剂量组（7mg/kg）、中剂量组（14mg/kg）和高剂量组（35mg/kg）。18 天后，通过从小鼠眼球采血。比较各组小鼠的红细胞（RBC）、血红蛋白（HGB）、中性粒细胞/淋巴细胞比值（NLR）、血小板（PLT）、血小板分布宽度（PDW）、纤维蛋白原（FIB）和 D-二聚体（D-D）水平。
获得的人参皂苷 Rb 含量达到 90.05%。该提取过程简单可靠。中剂量的人参皂苷 Rb 可显著提高 RBC 和 HGB 水平（P<0.05）。此外，人参皂苷 Rb 可显著降低 NLR、PLT、PDW、FIB 和 D-D（P<0.01）。
人参皂苷 Rb 可显著改善癌症小鼠的贫血和血液高凝状态。人参皂苷 Rb 是治疗癌症的一种有潜力的抗凝和抗贫血药物。
