Chen Guiping, Zeng Ling, Yan Feng, Liu Jinlong, Qin Mengqi, Wang Feifei, Zhang Xu
Affiliated Eye Hospital of Nanchang University, Jiangxi Clinical Research Center of Ophthalmic Disease, Jiangxi Provincial Key Laboratory for Ophthalmology, Nanchang, JX, China.
School of Pharmacy, Nanchang University, Nanchang, JX, China.
Front Pharmacol. 2022 Jul 14;13:919905. doi: 10.3389/fphar.2022.919905. eCollection 2022.
Aging-related retinal degeneration can manifest as decreased visual function due to damage to retinal structures and dysfunction in retinal homeostasis. Naringenin, a flavonoid, has beneficial effects in preventing cellular aging, preserving the functionality of photoreceptors, and slowing down visual function loss. However, the role and potential mechanism of naringenin in the aging mouse retina require further investigation. In this study, we evaluated the effects of naringenin on the aging eye using electroretinogram (ERG) and hematoxylin and eosin staining and explored its potential mechanism by western blotting. ERG showed that naringenin increased the amplitude of the a- and b-waves of scotopic 3.0, 10.0, and the a-wave amplitude of photopic 3.0 in the aging mouse retina. Furthermore, administration of naringenin prevented aging-induced retinal degeneration in the total retina, ganglion cell, inner plexiform layer, inner nuclear layer, and outer nuclear layer. The expression of mitochondrial fusion protein two was increased, OPA1 protein expression and the ratio of L-OPA1/S-OPA1 were unchanged, and dynamin-related protein one was decreased in the 12-month-old mice treated with naringenin compared with the 12-month-old mice treated with vehicle. Furthermore, the downregulation of age-related alterations in autophagy was significantly rescued in the aging mice by treatment with naringenin. Taken together, these results suggest that the oral administration of naringenin improves visual function, retinal structure, mitochondrial dynamics, and autophagy in the aging mouse retinas. Naringenin may be a potential dietary supplement for the prevention or treatment of aging-related retinal degeneration.
与衰老相关的视网膜变性可表现为由于视网膜结构受损和视网膜内环境稳态功能障碍导致的视觉功能下降。柚皮素是一种黄酮类化合物，在预防细胞衰老、维持光感受器功能以及减缓视觉功能丧失方面具有有益作用。然而，柚皮素在衰老小鼠视网膜中的作用和潜在机制仍需进一步研究。在本研究中，我们使用视网膜电图（ERG）和苏木精-伊红染色评估了柚皮素对衰老眼睛的影响，并通过蛋白质印迹法探讨了其潜在机制。ERG显示，柚皮素增加了衰老小鼠视网膜中暗视3.0、10.0时a波和b波的振幅以及明视3.0时a波的振幅。此外，给予柚皮素可预防衰老诱导的整个视网膜、神经节细胞、内网状层、内核层和外核层的视网膜变性。与给予赋形剂处理的12月龄小鼠相比，给予柚皮素处理的12月龄小鼠中线粒体融合蛋白2的表达增加，OPA1蛋白表达和L-OPA1/S-OPA1比值不变，动力相关蛋白1减少。此外，用柚皮素处理可显著挽救衰老小鼠中自噬相关的年龄相关变化的下调。综上所述，这些结果表明口服柚皮素可改善衰老小鼠视网膜的视觉功能、视网膜结构、线粒体动力学和自噬。柚皮素可能是预防或治疗与衰老相关的视网膜变性的潜在膳食补充剂。
