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低值鱼抗氧化肽的优化与鉴定：提取、胃肠消化与分级分离。

Optimization and Identification of Antioxidant Peptide from Underutilized : Extraction, Gastrointestinal Digestion, and Fractionation.

机构信息

Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China.

出版信息

Biomed Res Int. 2019 Aug 20;2019:6424651. doi: 10.1155/2019/6424651. eCollection 2019.

DOI:10.1155/2019/6424651
PMID:31531361
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6720044/
Abstract

DPPH• scavenging peptides (<3kDa) from underutilized protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of could be a potential source of antioxidative peptides through simulated gastrointestinal digestion.

摘要

采用超声提取、模拟胃肠消化水解和膜超滤分级等连续处理方法，从未充分利用的蛋白质中获得 DPPH•清除肽（<3kDa）。超声辅助提取的最佳条件为功率 800 W 的超声波处理 60 mL 1.0 mol L NaOH 和 2 g 藻类粉混合物 15 min。DPPH•清除活性与完整肽得率之间具有高度相关性（r=0.8146），表明其具有抗氧化能力。模拟消化试验表明，总肽具有极好的 DPPH•清除活性，为（86.5±10.1）%，与未消化样品的（46.8±6.5）%相比，有显著提高。经分级后，500-1000 Da 级分表现出最高的 DPPH•清除活性（81.2±4.0）%，由于消化作用增加了 1.5 倍。然后，采用 RPLC-Q Exactive HF 质谱仪对 500-1000 Da 级分进行分析，鉴定出 4 种新型肽，即 Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys、Ile-Ile-Tyr-Phe-Gln-Gly-Lys、Asn-Asp-Pro-Ser-Thr-Val-Lys 和 Thr-Val-Arg-Pro-Pro-Gln-Arg。根据这些氨基酸序列，疏水性残基占 56%，表明其具有较高的抗氧化活性。结果表明，可通过模拟胃肠消化从未充分利用的蛋白质中获得抗氧化肽，这为开发新的抗氧化肽源提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/42a85a49c7a4/BMRI2019-6424651.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/717c34d4b4f5/BMRI2019-6424651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/7a33c1d043c9/BMRI2019-6424651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/e6eba91491f1/BMRI2019-6424651.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/4a32503f64a6/BMRI2019-6424651.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/42a85a49c7a4/BMRI2019-6424651.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/717c34d4b4f5/BMRI2019-6424651.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/7a33c1d043c9/BMRI2019-6424651.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/e6eba91491f1/BMRI2019-6424651.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/4a32503f64a6/BMRI2019-6424651.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68e9/6720044/42a85a49c7a4/BMRI2019-6424651.005.jpg

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