• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

南极磷虾（）蛋白碱性蛋白酶水解物抗氧化肽的分离纯化、鉴定、活性评价及稳定性研究。

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill () Proteins.

机构信息

Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China.

National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

出版信息

Mar Drugs. 2021 Jun 17;19(6):347. doi: 10.3390/md19060347.

DOI:10.3390/md19060347
PMID:34204535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8235214/
Abstract

For utilizing the largest source of marine proteins, Antarctic krill () proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against HO-damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6-8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products.

摘要

为了利用最大的海洋蛋白质资源，分别使用胃蛋白酶、碱性蛋白酶、木瓜蛋白酶、胰蛋白酶和中性蛋白酶对南极磷虾蛋白进行脱脂和水解，五种水解产物中，碱性蛋白酶水解产物（EPAH）显示出最高的 DPPH 自由基（DPPH·）和羟基自由基（HO·）清除活性。通过超滤和色谱方法，从 EPAH 中纯化出 15 种抗氧化肽，并鉴定为 Asn-Gln-Met (NQM)、Trp-Phe-Pro-Met (WFPM)、Gln-Asn-Pro-Thr (QNPT)、Tyr-Met-Asn-Phe (YMNF)、Ser-Gly-Pro-Ala (SGPA)、Ser-Leu-Pro-Tyr (SLPY)、Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY)、Glu-Tyr-Glu-Ala (EYEA)、Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV)、Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI)、Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF)、Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ)、Ala-Phe-Leu-Trp-Asn (AFLWA)、Asn-Val-Pro-Asp-Met (NVPDM) 和 Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ)，分别使用蛋白质测序仪和 ESI/MS。在 15 种抗氧化肽中，SLPY、QYPPMQY 和 EYEA 对 DPPH·（EC 值分别为 1.18 ± 0.036、1.547 ± 0.150 和 1.372 ± 0.274 mg/mL）、HO·（EC 值分别为 0.826 ± 0.027、1.022 ± 0.058 和 0.946 ± 0.011 mg/mL）和超氧阴离子自由基（EC 值分别为 0.789 ± 0.079、0.913 ± 0.007 和 0.793 ± 0.056 mg/mL）具有最高的清除活性。此外，SLPY、QYPPMQY 和 EYEA 具有较强的还原能力、对 HO 损伤质粒 DNA 的保护能力和抑制脂质过氧化能力。此外，SLPY、QYPPMQY 和 EYEA 在低于 80°C 的温度、pH 值为 6-8 以及模拟 GI 消化 180 min 的条件下具有较高的稳定性。结果表明，南极磷虾蛋白碱性蛋白酶水解产物中的 15 种抗氧化肽，特别是 SLPY、QYPPMQY 和 EYEA，可能作为有效的抗氧化剂应用于食品和保健品中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/0d5882f77431/marinedrugs-19-00347-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2e65bf84594a/marinedrugs-19-00347-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/a2d6a5479870/marinedrugs-19-00347-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/92dec7f0f192/marinedrugs-19-00347-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/cb201c6bac43/marinedrugs-19-00347-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2634193f221e/marinedrugs-19-00347-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/435bb38553ae/marinedrugs-19-00347-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/57e01cfea06b/marinedrugs-19-00347-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/a088e3cbc44b/marinedrugs-19-00347-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/ed532966865f/marinedrugs-19-00347-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/643be23ace8e/marinedrugs-19-00347-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2c7e2dcd96b6/marinedrugs-19-00347-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/0d5882f77431/marinedrugs-19-00347-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2e65bf84594a/marinedrugs-19-00347-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/a2d6a5479870/marinedrugs-19-00347-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/92dec7f0f192/marinedrugs-19-00347-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/cb201c6bac43/marinedrugs-19-00347-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2634193f221e/marinedrugs-19-00347-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/435bb38553ae/marinedrugs-19-00347-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/57e01cfea06b/marinedrugs-19-00347-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/a088e3cbc44b/marinedrugs-19-00347-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/ed532966865f/marinedrugs-19-00347-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/643be23ace8e/marinedrugs-19-00347-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/2c7e2dcd96b6/marinedrugs-19-00347-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d575/8235214/0d5882f77431/marinedrugs-19-00347-g012.jpg

相似文献

1
Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill () Proteins.南极磷虾（）蛋白碱性蛋白酶水解物抗氧化肽的分离纯化、鉴定、活性评价及稳定性研究。
Mar Drugs. 2021 Jun 17;19(6):347. doi: 10.3390/md19060347.
2
The primary structure of human liver manganese superoxide dismutase.人肝脏锰超氧化物歧化酶的一级结构。
J Biol Chem. 1984 Oct 25;259(20):12595-601.
3
Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein.小鼠主要组织相容性复合体同种异体抗原的一级结构：H-2Kb糖蛋白氨基末端173个残基的氨基酸序列。
Biochemistry. 1980 Jan 22;19(2):306-15. doi: 10.1021/bi00543a009.
4
The primary structure of iron-superoxide dismutase from Photobacterium leiognathi.来自鱼发光杆菌的铁超氧化物歧化酶的一级结构。
J Biol Chem. 1987 Jan 25;262(3):1001-9.
5
The amino acid sequence of human chorionic gonadotropin. The alpha subunit and beta subunit.人绒毛膜促性腺激素的氨基酸序列。α亚基和β亚基。
J Biol Chem. 1975 Jul 10;250(13):5247-58.
6
Primary and tertiary structure of the principal human adenylate kinase.主要人类腺苷酸激酶的一级和三级结构
Eur J Biochem. 1976 Sep;68(1):281-90. doi: 10.1111/j.1432-1033.1976.tb10787.x.
7
Preparation, Identification, and Activity Evaluation of Eight Antioxidant Peptides from Protein Hydrolysate of Hairtail () Muscle.带鱼（）肌肉蛋白水解物中 8 种抗氧化肽的制备、鉴定及活性评价。
Mar Drugs. 2019 Jan 2;17(1):23. doi: 10.3390/md17010023.
8
Antioxidant Peptides from the Protein Hydrolysate of Spanish Mackerel () Muscle by in Vitro Gastrointestinal Digestion and Their In Vitro Activities.西班牙鲭鱼肌肉蛋白水解物经体外胃肠消化后的抗氧化肽及其体外活性。
Mar Drugs. 2019 Sep 12;17(9):531. doi: 10.3390/md17090531.
9
Complete amino acid sequence of copper-zinc superoxide dismutase from Drosophila melanogaster.黑腹果蝇铜锌超氧化物歧化酶的完整氨基酸序列。
Arch Biochem Biophys. 1985 Sep;241(2):577-89. doi: 10.1016/0003-9861(85)90583-1.
10
Eight antihypertensive peptides from the protein hydrolysate of Antarctic krill (Euphausia superba): Isolation, identification, and activity evaluation on human umbilical vein endothelial cells (HUVECs).八种从南极磷虾（Euphausia superba）蛋白水解物中分离得到的降压肽：鉴定及其对人脐静脉内皮细胞（HUVECs）活性的评价。
Food Res Int. 2019 Jul;121:197-204. doi: 10.1016/j.foodres.2019.03.035. Epub 2019 Mar 19.

引用本文的文献

1
Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Antarctic Krill Peptides with Multi-Bioactivities.海洋细菌金属蛋白酶A69在制备具有多种生物活性的南极磷虾肽中的潜力
Mar Drugs. 2025 May 24;23(6):226. doi: 10.3390/md23060226.
2
Review on bioactive peptides from Antarctic krill: From preparation to structure-activity relationship and tech-functionality.南极磷虾生物活性肽综述：从制备到构效关系及技术功能性
Curr Res Food Sci. 2025 May 24;10:101093. doi: 10.1016/j.crfs.2025.101093. eCollection 2025.
3
Bioactivity of Marine-Derived Peptides and Proteins: A Review.

本文引用的文献

1
Preparation process optimization, structural characterization and in vitro digestion stability analysis of Antarctic krill (Euphausia superba) peptides-zinc chelate.南极磷虾（Euphausia superba）肽-锌配合物的制备工艺优化、结构表征及体外消化稳定性分析。
Food Chem. 2021 Mar 15;340:128056. doi: 10.1016/j.foodchem.2020.128056. Epub 2020 Sep 25.
2
Chitin from Antarctic krill shell: Eco-preparation, detection, and characterization.南极磷虾壳来源的几丁质：生态制备、检测与表征。
Int J Biol Macromol. 2020 Dec 1;164:4125-4137. doi: 10.1016/j.ijbiomac.2020.08.244. Epub 2020 Sep 2.
3
The novel peptides ICRD and LCGEC screened from tuna roe show antioxidative activity via Keap1/Nrf2-ARE pathway regulation and gut microbiota modulation.
海洋来源的肽和蛋白质的生物活性：综述
Mar Drugs. 2025 Apr 4;23(4):157. doi: 10.3390/md23040157.
4
Effects of Hot-Air Drying Temperatures on Quality and Volatile Flavor Components of Cooked Antarctic krill ().热风干燥温度对熟制南极磷虾品质及挥发性风味成分的影响()。 （注：括号内原文内容缺失，所以译文括号处也无法准确完整翻译）
Foods. 2025 Mar 31;14(7):1221. doi: 10.3390/foods14071221.
5
Enhancement of Antioxidant Activity, Stability, and Structure of Heme-Peptides by L-Lysine.L-赖氨酸对血红素肽抗氧化活性、稳定性及结构的增强作用
Foods. 2025 Jan 9;14(2):192. doi: 10.3390/foods14020192.
6
E'jiao and Cubilose Formula Induced Antioxidant Activity and Improved Collagen Expression of Human Skin Fibroblasts During Oxidation Damage.阿胶与燕窝配方诱导人皮肤成纤维细胞在氧化损伤过程中的抗氧化活性并改善胶原蛋白表达。
J Cosmet Dermatol. 2025 Jan;24(1):e16604. doi: 10.1111/jocd.16604. Epub 2024 Nov 12.
7
Isolation and Characterization of Antioxidant Peptides from Dairy Cow () Placenta and Their Antioxidant Activities.奶牛胎盘抗氧化肽的分离、鉴定及其抗氧化活性
Antioxidants (Basel). 2024 Jul 29;13(8):913. doi: 10.3390/antiox13080913.
8
Bioactive peptides of marine organisms: Roles in the reduction and control of cardiovascular diseases.海洋生物的生物活性肽：在心血管疾病的减轻和控制中的作用。
Food Sci Nutr. 2024 Apr 23;12(8):5271-5284. doi: 10.1002/fsn3.4183. eCollection 2024 Aug.
9
Novel Angiotensin-Converting Enzyme-Inhibitory Peptides Obtained from : Preparation, Identification and Potential Antihypertensive Mechanism.新型血管紧张素转换酶抑制肽的获得：制备、鉴定及潜在的降压机制。
Biomolecules. 2024 May 15;14(5):581. doi: 10.3390/biom14050581.
10
Identification and Functional Validation of Two Novel Antioxidant Peptides in Saffron.藏红花中两种新型抗氧化肽的鉴定与功能验证
Antioxidants (Basel). 2024 Mar 20;13(3):378. doi: 10.3390/antiox13030378.
从金枪鱼鱼卵中筛选出的新型肽 ICRD 和 LCGEC 通过 Keap1/Nrf2-ARE 通路调节和肠道微生物群调节表现出抗氧化活性。
Food Chem. 2020 Oct 15;327:127094. doi: 10.1016/j.foodchem.2020.127094. Epub 2020 May 20.
4
Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates.鲭鱼（日本鲭）肌肉蛋白水解物中新型抗氧化肽的鉴定与表征
Food Chem. 2020 Apr 15;323:126809. doi: 10.1016/j.foodchem.2020.126809.
5
Purification and identification of antioxidant peptides from duck plasma proteins.鸭血浆蛋白抗氧化肽的分离鉴定。
Food Chem. 2020 Jul 30;319:126534. doi: 10.1016/j.foodchem.2020.126534. Epub 2020 Mar 2.
6
Antioxidant Peptides from the Protein Hydrolysate of Monkfish () Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by HO.鳕鱼（）肌肉蛋白水解物中的抗氧化肽：分离、鉴定及其对 HO 诱导的 HepG2 细胞损伤的细胞保护作用。
Mar Drugs. 2020 Mar 10;18(3):153. doi: 10.3390/md18030153.
7
Characterization and Antioxidant Activity of Collagen, Gelatin, and the Derived Peptides from Yellowfin Tuna () Skin.黄鳍金枪鱼（）皮胶原蛋白、明胶及其衍生肽的特性与抗氧化活性研究。
Mar Drugs. 2020 Jan 31;18(2):98. doi: 10.3390/md18020098.
8
Cytoprotective Effect of Antioxidant Pentapeptides from the Protein Hydrolysate of Swim Bladders of Miiuy Croaker ( ) against HO-Mediated Human Umbilical Vein Endothelial Cell (HUVEC) Injury.抗氧化五肽对 HO 介导的人脐静脉内皮细胞（HUVEC）损伤的保护作用。
Int J Mol Sci. 2019 Oct 31;20(21):5425. doi: 10.3390/ijms20215425.
9
Identification and Active Evaluation of Antioxidant Peptides from Protein Hydrolysates of Skipjack Tuna () Head.鲣鱼头蛋白水解物中抗氧化肽的鉴定与活性评价
Antioxidants (Basel). 2019 Aug 19;8(8):318. doi: 10.3390/antiox8080318.
10
High Fischer ratio oligopeptides determination from Antartic krill: Preparation, peptides profiles, and in vitro antioxidant activity.南极磷虾高 Fischer 比寡肽的测定：制备、肽谱及体外抗氧化活性。
J Food Biochem. 2019 May;43(5):e12827. doi: 10.1111/jfbc.12827. Epub 2019 Mar 21.