火棘提取物对氯化镉诱导的大鼠急性肾毒性的保护作用。
Protective roles of Pyracantha fortuneana extract on acute renal toxicity induced by cadmium chloride in rats.
作者信息
Ke Yixin, Yu Kaihang, Zeng Weiliang, Lian Guojun
机构信息
Graduate student, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation and writing.
Graduate student, Department of Health Inspection and Quarantine, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China. Technical procedures, acquisition of data.
出版信息
Acta Cir Bras. 2019 Sep 12;34(7):e201900706. doi: 10.1590/s0102-865020190070000006.
PURPOSE
To investigate the protective roles of pyracantha fortune fruit extract (PFE) on acute renal toxicity induced by cadmium chloride (CdCl2) in rats.
METHODS
Rats were pretreated with PFE and consecutively injected with CdCl2 (6.5 mg/kg) for 5 days.
RESULTS
The concentration of Cd, kidney weight, malondialdehyde (MDA), and nitric oxide (NO) production were remarkably increased in CdCl2 group as well as the levels of plasma uric acid, urea, and creatinine (P < 0.001). However, the body weight and glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione peroxidase (GR) levels were markedly reduced by CdCl2 treatment (P < 0.001). Histological manifestations of renal tissue showed severely adverse changes. Moreover, CdCl2 treatment significantly decreased the B-cell lymphoma-2 (Bcl-2) expression while increased the Bcl-2-Associated X Protein (Bax), tumor necrosis factor-α (TNF-α) expression (P < 0.001). Additionally, the expression of Nrf2/Keap 1 related proteins Keap-1 gained a significant increase (P < 0.001), whereas the Nrf2, HO-1, γ-GCS, GSH-Px and NQO1 expression decreased by CdCl2 treatment (P < 0.05). These rats were pretreated with PFE to improve the changes caused by CdCl2 treatment.
CONCLUSION
PFE could protect the kidney against acute renal toxicity induced by CdCl2.
目的
探讨火棘果提取物（PFE）对氯化镉（CdCl2）诱导的大鼠急性肾毒性的保护作用。
方法
大鼠经PFE预处理，然后连续5天注射CdCl2（6.5mg/kg）。
结果
CdCl2组镉浓度、肾脏重量、丙二醛（MDA）和一氧化氮（NO）生成量显著增加，血浆尿酸、尿素和肌酐水平也显著升高（P<0.001）。然而，CdCl2处理使体重以及谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）水平显著降低（P<0.001）。肾组织的组织学表现显示出严重的不良变化。此外，CdCl2处理显著降低了B细胞淋巴瘤-2（Bcl-2）的表达，同时增加了Bcl-2相关X蛋白（Bax）、肿瘤坏死因子-α（TNF-α）的表达（P<0.001）。此外，Nrf2/Keap 1相关蛋白Keap-1的表达显著增加（P<0.001），而CdCl2处理使Nrf2、HO-1、γ-GCS、GSH-Px和NQO1的表达降低（P<0.05）。用PFE预处理这些大鼠可改善CdCl2处理引起的变化。
结论
PFE可保护肾脏免受CdCl2诱导的急性肾毒性。
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