The Research Institute of Obstetrics, Gynecology and Reproductology Named after D.O. Ott , St. Petersburg , Russia.
Scandinavia Clinics , St. Petersburg , Russia.
Gynecol Endocrinol. 2019;35(sup1):49-55. doi: 10.1080/09513590.2019.1653563.
The contemporary world despite its enough developed medicine and generally highly enlightened population faces a great problem of vitamin, micro-element and nutrient deficiency turning to become the XXI century pandemic. Along with that significant growth of interest can be seen towards vitamin D importance for reproductive physiology. The fact is that vitamin D receptors (VDR) have been detected in women's ovarium tissue, fallopian tubes, decidua and placenta. Some recent years studies have proven that vitamin D may act as immune regulator during implantation. During early pregnancy the trophoblast release vitamin D, which produces anti-inflammatory reaction and also induce decidual tissue growth for successive pregnancy. It was a comparison between the expression of Vitamin D and VDR in chorionic villous in cases of normal pregnancy and missed abortion groups. 64 samples of chorionic villous were taken: 32 from missed abortion and 32 from the induced abortion group. Abortive material was taken from two groups of women residing in North-West region of Russia: missed abortion and pregnancy terminated at woman's wish (induced abortion); 6-12 weeks of gestation, singleton pregnancy. Immune histochemical examination showed homogenous distribution of vitamin D and VDR expression in syncytiotrophoblasts, cytotrophoblasts and chorion villus stroma.Vitamin D expression relative area was 10,3% which is statistically different from the induced abortion group - 15,4% (p<0,01). VDR expression analysis showed its homogenous distribution in chorionic villus structures in both groups. High VDR expression was detected in chorion villus stromal components. In missed abortion group, the morphometry results showed distinctly lower relative area of vitamin D expression against the comparison group (35,9 ± 1,8; 56,1 ± 2,4 p < 0,01). Also in missed abortion group, positively significant correlation has been determined between the level of vitamin D in blood and VDR relative area expression (r = 0,412). In missed abortion group, definite vitamin D and VDR expression decrease was detected compared to the induced abortion group. The results witness vitamin D importance for pregnancy progress.
当代世界尽管医学已经相当发达，人口普遍文化水平也很高，但仍面临着一个严重的问题，即维生素、微量元素和营养素缺乏症，这已成为 21 世纪的一种大流行病。与此同时，人们对维生素 D 对生殖生理学重要性的兴趣显著增加。事实上，维生素 D 受体 (VDR) 已在女性卵巢组织、输卵管、蜕膜和胎盘组织中检测到。近年来的一些研究已经证明，维生素 D 可能在着床过程中充当免疫调节剂。在早孕期间，滋养层细胞释放维生素 D，产生抗炎反应，并诱导蜕膜组织生长，以维持后续妊娠。这是在正常妊娠和难免流产组的绒毛组织中比较维生素 D 和 VDR 的表达。从居住在俄罗斯西北部的两组妇女中采集了 64 个绒毛样本：32 个来自难免流产，32 个来自人工流产。从两组妇女中采集流产材料：难免流产和妇女意愿终止妊娠（人工流产）；妊娠 6-12 周，单胎妊娠。免疫组织化学检查显示维生素 D 和 VDR 在合体滋养层细胞、细胞滋养层和绒毛间质中的表达均匀分布。维生素 D 表达相对面积为 10.3%，与人工流产组（15.4%）有统计学差异（p<0.01）。VDR 表达分析显示，两组绒毛结构中的表达均匀分布。在绒毛间质成分中检测到高 VDR 表达。在难免流产组中，形态计量学结果显示，维生素 D 表达的相对面积明显低于对照组（35.9±1.8；56.1±2.4 p<0.01）。在难免流产组中，还确定了血液中维生素 D 水平与 VDR 相对面积表达之间存在显著正相关（r=0.412）。与人工流产组相比，难免流产组中维生素 D 和 VDR 的表达明显下降。这些结果证明了维生素 D 对妊娠进展的重要性。
