Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, H 8/4, Islamabad 44000, Pakistan.
Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Nat Prod Res. 2021 Jul;35(13):2257-2262. doi: 10.1080/14786419.2019.1667347. Epub 2019 Sep 19.
Lindl. ex A. Camus nuts have important folklore uses, assessed for underexplored biological potential. Nuts galls or cores and coats were utilized for the preparation of extracts using 14 solvent systems. Antioxidant, antimicrobial, cytotoxic and enzyme inhibition assays along with phytochemical profiling was carried out. Distilled water cores extract demonstrated maximum percent yield, phenolics content and total antioxidant capacity. Methanol: ethyl acetate cores extract showed maximum flavonoids content, total reducing power and protein kinase inhibition. Highest percentage radical scavenging and brine shrimp lethality was revealed by acetone: distilled water cores extract. Ethyl acetate cores extract indicated maximum α-amylase inhibition. Methanol: water coats extract showed substantial leishmanial growth inhibition. -Hexane and chloroform coats extracts showed maximum cytotoxicity against HepG2 and THP-1 cell lines, respectively. Polyphenols quantified through RP-HPLC analysis were quercetin, pyrocatechol, gallic acid, catechin and chlorogenic acid ranging from 0.003 ± 0.001 to 1.785 ± 0.5 μg/mg extract.
林德尔。经 A. 卡缪坚果具有重要的民间用途，评估其未被充分探索的生物潜力。坚果瘿或核仁和外壳被用于使用 14 种溶剂系统制备提取物。进行了抗氧化、抗菌、细胞毒性和酶抑制测定以及植物化学分析。蒸馏水核仁提取物表现出最大的产率百分比、多酚含量和总抗氧化能力。甲醇：乙酸乙酯核仁提取物显示出最大的类黄酮含量、总还原能力和蛋白激酶抑制。丙酮：蒸馏水核仁提取物显示出最高的自由基清除率和卤虫致死率。乙酸乙酯核仁提取物显示出最大的α-淀粉酶抑制活性。甲醇：水壳提取物显示出对利什曼原虫生长的显著抑制作用。-己烷和氯仿壳提取物对 HepG2 和 THP-1 细胞系表现出最大的细胞毒性。通过反相高效液相色谱分析定量的多酚为槲皮素、邻苯二酚、没食子酸、儿茶素和绿原酸，范围为 0.003 ± 0.001 至 1.785 ± 0.5 μg/mg 提取物。
