Department of Pharmacognosy, Faculty of Pharmacy & Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Fitoterapia. 2023 Oct;170:105628. doi: 10.1016/j.fitote.2023.105628. Epub 2023 Jul 28.
Crude extracts prepared from aerial parts and nut galls of Quercus floribunda Lindl. Ex. A. Camus were evaluated for phytochemical screening, in vitro antioxidant, and in vivo analgesic, anti-inflammatory and antipyretic activities. Various solvents including methanol (M), acetone (A), distilled water (DW), distilled water + methanol (DWM) were used for extraction. Highest total phenolic (66.9 ± 0.05 μg GAE/mgE) and flavonoid content (38.4 ± 0.72 μg QE/mgE) were measured in QFAA extract by colorimetric methods. Cumulative maximum concentrations of polyphenols were quantified in QFMG, QFAA, and QFMA extracts i.e. 19.036, 15. 574 and 11.647 μg/mg of extract by RP-HPLC analysis. From aerial parts extracts, apentacyclic tritepenoid, glutinol was isolated using column chromatography techniques and structure was elucidated using spectroscopic techniques. QFDWMA (205.5 ± 0.56 μg AAE/mg of extract) showed highest total reducing power while highest total antioxidant capacity (207.1 ± 0.49 AAE/mg of extract) and free radical scavenging potential (96.1 ± 0.42%) were observed in QFAA extract. QFAA extract showed significant (p ≤ 0.001) analgesic potential in different pain models i.e. hot plate method, cold plate method, Haffner's tail clip method and acetic acid induced writhing assay having 50.20%, 62.07%, 57.26% and 70.49% analgesia respectively at 300 mg/kg. QFAA extract showed maximum anti-inflammatory activity in croton oil induced edema (68.83%) and in carrageenan induced paw edema models (72.32%) at 300 mg/kg concentration. QFAA extract markedly reduced the rectal temperature at 300 mg/kg concentration, in brewer's yeast induced pyrexia model. Detailed investigations can be executed in future to determine the molecular mechanisms of these pharmacological attributes.
从黄连木的地上部分和虫瘿中提取的粗提取物，经过评估，具有植物化学筛选、体外抗氧化、体内镇痛、抗炎和退热活性。使用了包括甲醇（M）、丙酮（A）、蒸馏水（DW）、蒸馏水+甲醇（DWM）在内的各种溶剂进行提取。通过比色法测定，QFAA 提取物中的总酚（66.9±0.05μgGAE/mgE）和类黄酮含量（38.4±0.72μgQE/mgE）最高。通过 RP-HPLC 分析，在 QFMG、QFAA 和 QFMA 提取物中定量累积的多酚最大浓度分别为 19.036、15.574 和 11.647μg/mg 提取物。从地上部分提取物中，使用柱层析技术分离出五环三萜类化合物，glutinol，并通过光谱技术阐明了其结构。QF-DWMA（205.5±0.56μgAAE/mg 提取物）表现出最高的总还原能力，而 QFAA 提取物表现出最高的总抗氧化能力（207.1±0.49AAE/mg 提取物）和自由基清除能力（96.1±0.42%）。QFAA 提取物在不同的疼痛模型中表现出显著的（p≤0.001）镇痛潜力，即在热板法、冷板法、Haffner 尾巴夹法和醋酸诱导扭体法中，分别有 50.20%、62.07%、57.26%和 70.49%的镇痛作用，剂量为 300mg/kg。QFAA 提取物在巴豆油诱导的水肿（68.83%）和角叉菜胶诱导的爪水肿模型中表现出最大的抗炎活性，剂量为 300mg/kg。QFAA 提取物在 300mg/kg 浓度的啤酒酵母诱导发热模型中显著降低直肠温度。在未来可以进行详细的研究，以确定这些药理特性的分子机制。
