Shamoun Simon Francis, Hammett Craig, Sumampong Grace, Li Xiang, Garbelotto Matteo
Pacific Forestry Centre, Canadian Forest Service, Natural Resources Canada, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver BC V6T 1Z4, Canada.
Pathogens. 2019 Sep 18;8(3):156. doi: 10.3390/pathogens8030156.
is a species complex of pathogenic white-rot wood decay fungi which cause root and butt rot in conifer and hardwood species across the Northern hemisphere. Annual losses to forest managers are valued in the billions of dollars, due to tree mortality, reduction in timber yield, and wood decay. In North America, and have a partially overlapping host and geographic range, cause similar disease symptoms and produce similar fruiting bodies, making discrimination between the two of them often difficult. We developed two sets of primers that bind specifically to conserved, but species-specific portions of glyceraldehyde 3-phosphate dehydrogenase and elongation factor 1α alleles. The method is sensitive enough to detect either species from infected wood. Analysis of North American isolates has further clarified the distribution of both species on this continent, including the detection of for the first time on ponderosa pine () and eastern white pine () in British Columbia. This method has the potential to be a valuable tool for the detection of the pathogen in exported/imported wood products, as well as for the further identification and assessment of the distribution of North American species.
是一种致病性白腐木材腐朽真菌的复合种，可导致北半球针叶树和阔叶树种的根腐和干基腐病。由于树木死亡、木材产量下降和木材腐朽，森林管理者每年的损失达数十亿美元。在北美， 和 有部分重叠的寄主和地理范围，引起相似的病害症状并产生相似的子实体，这使得区分它们两者往往很困难。我们开发了两组引物，它们特异性结合甘油醛-3-磷酸脱氢酶和延伸因子1α等位基因的保守但物种特异性部分。该方法灵敏度足够高，能够从受感染木材中检测出任何一种真菌。对北美分离株的分析进一步明确了这两个物种在该大陆的分布，包括首次在不列颠哥伦比亚省的黄松（）和东部白松（）上检测到 。该方法有可能成为检测进出口木制品中病原体的有价值工具，以及进一步鉴定和评估北美 物种分布的工具。
