State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, Guangdong, China.
State Key Laboratory of Nuclear Resources and Environment, East China University of Technology, Nanchang, 330013, China.
Antonie Van Leeuwenhoek. 2020 Jan;113(1):147-154. doi: 10.1007/s10482-019-01324-1. Epub 2019 Sep 21.
A Gram-stain-negative, rod-shaped and aerobic bacterium, designated CL12, was isolated from roots of Glycine max (Linn. Merr.) collected from an experimental field in the campus of South China Agricultural University, PR China (22°5846″S, 110°51'10″E). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CL12 belongs to the genus Rhizobium, closely related to Rhizobium wuzhouense W44 (99.3%), followed by Rhizobium rosettiformans W3 (98.0%) and Rhizobium ipomoeae Shin9-1 (97.9%). The results of analysis of sequences of four housekeeping genes (recA, atpD, rpoB and glnA) also revealed strain CL12 to be closely related to R. wuzhouense W44 with the similarities 91.0%, 95.0%, 94.2% and 90.5%, respectively. The major fatty acid of strain CL12 was Summed Feature 8 (Cω7c and/or Cω6c). Strain CL12 had not the nodulation genes (nodC and nodA) and nitrogenase reductase gene (nifH), and could not cause formation of nodule on soybean. The draft genome size of strain CL12 was 4.84 Mbp with a genomic DNA G + C content of 61.1 mol%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) of strain CL12 and R. wuzhouense W44 were 27.4% and 84.7%, respectively. Based on genomic, phenotypic and phylogenetic analysis, strain CL12 is suggested to represent a new species of the genus Rhizobium, for which the name Rhizobium glycinendophyticum sp. nov. is proposed. The type strain is CL12 (=GDMCC 1.1597 = KACC 21281).
一株革兰氏阴性、杆状、需氧细菌，被命名为 CL12，是从中国华南农业大学校园实验田的大豆（Linn. Merr.）根部分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CL12 属于根瘤菌属，与梧州根瘤菌 W44（99.3%）密切相关，其次是花椰菜根瘤菌 W3（98.0%）和甘薯根瘤菌 Shin9-1（97.9%）。四个看家基因（recA、atpD、rpoB 和 glnA）序列分析的结果也表明，菌株 CL12 与 R. wuzhouense W44 密切相关，相似度分别为 91.0%、95.0%、94.2%和 90.5%。菌株 CL12 的主要脂肪酸为 Summed Feature 8（Cω7c 和/或 Cω6c）。菌株 CL12 没有结瘤基因（nodC 和 nodA）和氮还原酶基因（nifH），不能在大豆上形成根瘤。菌株 CL12 的基因组大小为 4.84 Mbp，基因组 DNA G+C 含量为 61.1 mol%。菌株 CL12 和 R. wuzhouense W44 的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）分别为 27.4%和 84.7%。基于基因组、表型和系统发育分析，菌株 CL12 被建议代表根瘤菌属的一个新种，命名为根瘤菌内生大豆（Rhizobium glycinendophyticum sp. nov.）。模式菌株为 CL12（=GDMCC 1.1597=KACC 21281）。
