State Key Laboratory of Biobased Transportation Fuel Technology, Ocean College, Zhejiang University, Room 377, Marine Science Building, No.1 Zheda Road, Dinghai District, Zhoushan, 316021, Zhejiang, China.
Hainan Institute of Zhejiang University, Sanya, 572000, China.
Antonie Van Leeuwenhoek. 2024 Aug 12;117(1):112. doi: 10.1007/s10482-024-02010-7.
A Gram-stain-negative, light khaki, strictly aerobic, rod-shaped, motile via multiple flagella, and catalase- and oxidase-positive bacterium, designated as SSM4.3, was isolated from the seaweed of Gouqi Island in the East China Sea. The novel isolate grows at 0-5.0% NaCl concentrations (w/v) (optimum 1%), pH 5.0-9.0 (optimum pH 7.0), and 15-37 °C (optimum 30 °C). The 16S rRNA gene sequences-based phylogeny indicates that the novel marine isolate belongs to the family Rhizobiaceae and that it shared the greatest sequence similarity (98.9%) with Peteryoungia rhizophila CGMCC 1.15691. This classification was also supported by phylogenetic analysis using core genes. The predominant fatty acids (≥ 10%) of the strain were identified as C ω7c/C ω6c. Q-10 was identified as the major isoprenoid quinone, with trace levels of Q-9 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The complete genome size of strain SSM4.3 is 4.39 Mb with a DNA G+C content of 61.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain SSM4.3 and its closely related representatives were 74.80-86.93%, 20.00-32.30%, and 70.30-91.52%, respectively. Phylogenetic analysis, grounded on the core genes, reveals the evolutionary relationship between SSM4.3 and other Peteryoungia strains. Pan-genomics analysis of 8 previously classified Peteryoungia species and SSM4.3 revealed their unique genetic features and functions. Overall, strain SSM4.3 was considered to be a new species of the Peteryoungia genus; the name Peteryoungia algae sp. nov. has been proposed, with type strain SSM4.3 (= LMG 32561 = MCCC 1K07170).
一株革兰氏阴性、浅土黄色、严格需氧、杆状、通过多根鞭毛运动、过氧化氢酶和氧化酶阳性的细菌，被命名为 SSM4.3，从东海枸杞岛的海藻中分离得到。该新分离株在 0-5.0%（w/v）NaCl 浓度（最佳 1%）、pH5.0-9.0（最佳 pH7.0）和 15-37°C（最佳 30°C）下生长。基于 16S rRNA 基因序列的系统发育分析表明，该新型海洋分离株属于根瘤菌科，与佩特里氏菌（Peteryoungia） rhizophila CGMCC 1.15691 的序列相似度最高（98.9%）。这种分类也得到了基于核心基因的系统发育分析的支持。该菌株的主要脂肪酸（≥10%）为 C ω7c/C ω6c。Q-10 被鉴定为主要的异戊烯醌，存在痕量的 Q-9。主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。SSM4.3 菌株的全基因组大小为 4.39 Mb，G+C 含量为 61.3%。与近缘代表菌株的基因组相比，SSM4.3 菌株的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性分别为 74.80-86.93%、20.00-32.30%和 70.30-91.52%。基于核心基因的系统发育分析揭示了 SSM4.3 与其他佩特里氏菌菌株之间的进化关系。对 8 种已分类的佩特里氏菌种和 SSM4.3 的泛基因组分析揭示了它们独特的遗传特征和功能。总的来说，SSM4.3 菌株被认为是佩特里氏菌属的一个新种，提议将其命名为 Peteryoungia algae sp. nov.，其模式菌株为 SSM4.3（=LMG 32561=MCCC 1K07170）。
