National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra, 411007, India.
Post-Graduate Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Vadtal Road, Bakrol, Anand, Gujarat, 388 315, India.
Arch Microbiol. 2021 Aug;203(6):3591-3604. doi: 10.1007/s00203-021-02349-9. Epub 2021 May 9.
A novel bacterial strain designated as ADMK78 was isolated from the saline desert soil. The cells were rod-shaped, Gram-stain-negative, and non-motile. The strain ADMK78 grows best at 28 °C. Phylogeny of 16S rRNA gene placed the strain ADMK78 with the members of genera Ciceribacter and Rhizobium, while the highest sequence similarity was with Rhizobium wuzhouense W44 (98.7%) and Rhizobium ipomoeae shin9-1 (97.9%). Phylogenetic analysis based on 92 core-genes extracted from the genome sequences and average amino acid identity (AAI) revealed that the strain ADMK78 forms a distinct cluster including five species of Rhizobium, which is separate from the cluster of the genera Rhizobium and Ciceribacter. We propose re-classification of Rhizobium ipomoeae, R. wuzhouense, R. rosettiformans and R. rhizophilum into the novel genus Peteryoungia. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of ADMK78 were less than 82 and 81%, respectively, among all type strains included in the genus Peteryoungia. The strain ADMK78 showed differences in physiological, phenotypic, and protein profiles estimated by MALDI-TOF MS to its closest relatives. Based on the phenotypic, chemotaxonomic properties, and phylogenetic analyses, the strain ADMK78 represents a novel species, Peteryoungia desertarenae sp. nov. The type strain is ADMK78 (= MCC 3400; KACC 21383; JCM 33657). We also proposed the reclassification of Rhizobium daejeonense, R. naphthalenivorans and R. selenitireducens, into the genus Ciceribacter, based on core gene phylogeny and AAI values.
一株从盐漠土壤中分离到的新的细菌菌株 ADMK78 被命名为。细胞呈杆状，革兰氏阴性，不运动。菌株 ADMK78 在 28°C 时生长最好。16S rRNA 基因的系统发育将菌株 ADMK78 与 Ciceribacter 和 Rhizobium 属的成员放在一起，而与 Rhizobium wuzhouense W44（98.7%）和 Rhizobium ipomoeae shin9-1（97.9%）的序列相似度最高。基于从基因组序列中提取的 92 个核心基因和平均氨基酸同一性（AAI）的系统发育分析表明，菌株 ADMK78 形成了一个独特的聚类，包括 5 种 Rhizobium 物种，与 Rhizobium 和 Ciceribacter 属的聚类分开。我们建议将 Rhizobium ipomoeae、R. wuzhouense、R. rosettiformans 和 R. rhizophilum 重新分类为新属 Peteryoungia。ADMK78 与属内所有模式菌株的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值均小于 82%和 81%。ADMK78 与其最接近的亲缘关系在生理、表型和 MALDI-TOF MS 估计的蛋白质图谱方面存在差异。基于表型、化学分类学特性和系统发育分析，菌株 ADMK78 代表了一个新的物种，Peteryoungia desertarenae sp. nov。模式菌株为 ADMK78（= MCC 3400；KACC 21383；JCM 33657）。我们还根据核心基因系统发育和 AAI 值建议将 Rhizobium daejeonense、R. naphthalenivorans 和 R. selenitireducens 重新分类为 Ciceribacter 属。
