Lilaj Bledar, Dauti Rinet, Agis Hermann, Schmid-Schwap Martina, Franz Alexander, Kanz Fabian, Moritz Andreas, Schedle Andreas, Cvikl Barbara
Department of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
Department of Prosthodontics, University Clinic of Dentistry, Medical University of Vienna, Vienna, Austria.
Front Physiol. 2019 Aug 21;10:919. doi: 10.3389/fphys.2019.00919. eCollection 2019.
The aim of this study was to evaluate the effect of bleaching agents containing different concentrations of hydrogen peroxide (HP) on color-change and on enamel-surface in bovine teeth. Furthermore the influence on cell viability and proliferation was investigated. Two hundred and forty teeth were randomly assigned into four groups (home bleaching ≤6%, in-office bleaching ≤6%, in-office bleaching > 6% HP, and control group). Bleaching was performed after artificial staining and the bleached index (BI) as well as the whiteness index (WI ) was measured at several time points. Chemical analysis for HP concentrations and the pH of the bleaching products was done. Furthermore, enamel surfaces of randomly selected specimens were analyzed using scanning electron microscopy (SEM) and cytotoxicity of the tested bleaching products was evaluated using dental pulp cells (DPCs) and L929 cells. A statistically significant whitening effect was observed in almost all products. As expected all investigated products resulted in decreased cell viability, however, with different values of LC50 (median lethal concentration). SEM analysis showed an analog of enamel alterations with decreasing pH, increasing exposure time, and increasing HP concentration. Bleaching agents containing a low HP concentration are considered to be effective and to have less damaging effects on enamel and tested cells.
本研究的目的是评估不同浓度过氧化氢（HP）的漂白剂对牛牙颜色变化和牙釉质表面的影响。此外，还研究了其对细胞活力和增殖的影响。240颗牙齿被随机分为四组（家庭漂白≤6%、诊室漂白≤6%、诊室漂白>6%HP以及对照组）。人工染色后进行漂白，并在多个时间点测量漂白指数（BI）和白度指数（WI）。对HP浓度和漂白产品的pH值进行了化学分析。此外，使用扫描电子显微镜（SEM）对随机选择的样本的牙釉质表面进行了分析，并使用牙髓细胞（DPC）和L929细胞评估了测试漂白产品的细胞毒性。几乎所有产品都观察到了具有统计学意义的美白效果。正如预期的那样，所有研究产品都导致细胞活力下降，然而，其半数致死浓度（LC50）的值不同。SEM分析显示，随着pH值降低、暴露时间增加和HP浓度增加，牙釉质出现类似的改变。含低HP浓度的漂白剂被认为是有效的，并且对牙釉质和测试细胞的损害较小。
