Liu Jiangjun, Liu Zhongqiang, Zhu Yuanqi, Dong Bingzi, Cai Zheng, Liang Qing, Wang Kejia
Department of Basic Medical Sciences, School of Medicine, Xiamen University, Xiamen, China.
Department of Orthopedic Surgery, Qilu Hospital of Shandong University, Shandong, China.
Front Microbiol. 2019 Aug 30;10:2012. doi: 10.3389/fmicb.2019.02012. eCollection 2019.
T cells represent a subset of lymphocytes characterized by immunosurveillance and immunoregulation function. Peripheral blood mononuclear cells (PBMCs) are enriched in T cells, which exert critical antimicrobial roles in infectious diseases. High-throughput sequencing of the T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Flow cytometry was used to analyse the distribution of αβ/γδ T cells and their CD69, IFN-γ/IL-17 expression from PBMCs. Here, we utilized next-generation sequencing (NGS) to detect the complementarity determining region 3 (CDR3) of TCRβ (TRB) and TCRδ (TRD) chain after methicillin-resistant (MRSA) infection. Our data demonstrated a significant increase in the activation of αβ and γδ T cells after MRSA infection. Simultaneously, significantly high CDR3 amino acid (AA) diversity and markedly reconstituted TCR immune repertoires were observed after MRSA infection. Finally, we identified several MRSA-specific initial CDR3 AA motifs after MRSA infection. Our work reveals the profiles of TRB and TRD immune repertoires in response to MRSA and demonstrates a reconstitution of the TCR immune repertoire after MRSA infection.
T细胞是淋巴细胞的一个亚群，其特征在于具有免疫监视和免疫调节功能。外周血单个核细胞（PBMC）富含T细胞，它们在传染病中发挥关键的抗菌作用。T细胞受体（TCR）的高通量测序为监测免疫微环境提供了深入的见解。流式细胞术用于分析PBMC中αβ/γδ T细胞的分布及其CD69、IFN-γ/IL-17的表达。在此，我们利用下一代测序（NGS）检测耐甲氧西林金黄色葡萄球菌（MRSA）感染后TCRβ（TRB）和TCRδ（TRD）链的互补决定区3（CDR3）。我们的数据表明，MRSA感染后αβ和γδ T细胞的活化显著增加。同时，在MRSA感染后观察到显著高的CDR3氨基酸（AA）多样性和明显重构的TCR免疫库。最后，我们在MRSA感染后鉴定出几个MRSA特异性的初始CDR3 AA基序。我们的工作揭示了TRB和TRD免疫库对MRSA的反应概况，并证明了MRSA感染后TCR免疫库的重构。
