首次分子调查显示伊朗胡齐斯坦省农村犬类感染率很高。
First Molecular Survey on Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran.
作者信息
Hamidinejat Hossein, Bahrami Somayeh, Mosalanejad Bahman, Pahlavan Sharareh
机构信息
Department of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
出版信息
Iran J Parasitol. 2019 Apr-Jun;14(2):297-302.
BACKGROUND
Anaplasmosis due to is an important tick-borne zoonotic disease, which affects dogs, horses, cattle and human as well. This study aimed to probe the existence of this organism by means of molecular biology techniques for the first time in rural dogs of Khuzestan province, Southwestern Iran.
METHODS
During Sep 2014 to Apr 2015 blood samples of 103 apparently healthy rural dogs (60 males) were collected for detection by light microscopical examination of Giemsa stained slides and Nested PCR on a fragment of 16S rRNA gene.
RESULTS
From the examined slides, 11.65% were positive for while 57.28% of infection was revealed by Nested PCR method. There was no statistical difference between ages and sexes of dogs and infection in molecular survey of .
CONCLUSION
Molecular prevalence of was noticeably high. It may cause the incidence of disease in human population.
背景
由[病原体名称未给出]引起的无形体病是一种重要的蜱传人畜共患病，可感染犬、马、牛以及人类。本研究旨在首次通过分子生物学技术在伊朗西南部胡齐斯坦省的乡村犬中探究该病原体的存在情况。
方法
在2014年9月至2015年4月期间，采集了103只外观健康的乡村犬（60只为雄性）的血样，通过对吉姆萨染色玻片进行光学显微镜检查以及对16S rRNA基因片段进行巢式PCR来检测[病原体名称未给出]。
结果
在所检查的玻片样本中，[病原体名称未给出]阳性率为11.65%，而巢式PCR方法检测到的感染率为57.28%。在对[病原体名称未给出]的分子检测中，犬的年龄和性别与感染情况之间无统计学差异。
结论
[病原体名称未给出]的分子流行率显著较高。它可能导致人群中疾病的发生。
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