Fukui Yuichi, Inokuma Hisashi
Komachi Animal Hospital.
United Graduate School of Veterinary Science, Gifu University.
Jpn J Infect Dis. 2019 May 23;72(3):168-172. doi: 10.7883/yoken.JJID.2018.470. Epub 2019 Jan 31.
We evaluated the prevalence of Anaplasma infection in 332 dogs from Ibaraki, Japan, using serological and molecular methods. An immunofluorescence antibody assay against Anaplasma phagocytophilum indicated that 7 of the 328 serum samples tested (2.1%) were positive for A. phagocytophilum. Screening by polymerase chain reaction (PCR) analysis demonstrated that 8 of the 331 peripheral blood samples tested (2.4%) were positive for Anaplasmataceae. Phylogenetic analysis of the partial 16S rRNA sequence of the PCR amplicons revealed that 6 sequences were most similar to the 16S rRNA sequence of a Wolbachia sp., and the remaining 2 to A. bovis. Further analysis by A. phagocytophilum-specific nested PCR demonstrated that 1 dog infected with A. bovis was also positive for A. phagocytophilum. This is the first study to report the dual infection of a dog in Japan with A. bovis and A. phagocytophilum.
我们采用血清学和分子学方法，对来自日本茨城县的332只犬进行了无形体感染率评估。针对嗜吞噬细胞无形体的免疫荧光抗体检测表明，在检测的328份血清样本中，有7份（2.1%）对嗜吞噬细胞无形体呈阳性反应。通过聚合酶链反应（PCR）分析进行筛查显示，在检测的331份外周血样本中，有8份（2.4%）对无形体科呈阳性反应。对PCR扩增产物的部分16S rRNA序列进行系统发育分析发现，6个序列与一种沃尔巴克氏体属细菌的16S rRNA序列最为相似，其余2个与牛无形体的序列相似。通过嗜吞噬细胞无形体特异性巢式PCR进一步分析表明，1只感染牛无形体的犬对嗜吞噬细胞无形体也呈阳性反应。这是日本首次报道犬同时感染牛无形体和嗜吞噬细胞无形体的研究。
