Department of Pharmacy, The Second Hospital of Shandong University, Jinan, China.
Department of Pharmacy, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
J Microencapsul. 2019 Dec;36(8):728-737. doi: 10.1080/02652048.2019.1671908. Epub 2019 Oct 7.
To synthesise HSA-RB-DOX nanoparticles, measure its characteristics and preliminarily evaluate its anti-cancer effects. Doxorubicin (DOX) and Rose Bengal (RB) were co-delivered using albumin as a carrier. HSA-RB-DOX nanoparticles were prepared by RB-induced self-assembly of albumin. Its characteristics were measured and anti-cancer effects were tested in MCF-7 cells and tumour-bearing mice. HSA-RB-DOX nanoparticle with a mean size of 42 nm was stable in different medium and behaved controlled release characteristic. It was well took in MCF-7 cells and inhibited MCF-7 cells proliferation by inducing reactive oxygen species (ROS) production. It retained a much higher blood concentration up to 12 h and accumulated more in tumour tissues. In tumour-bearing mice, HSA-RB-DOX nanoparticles inhibited tumour growth and even decreased its volume from 100 to 50 mm, with barely no influence on body weight. HSA-RB-DOX nanoparticles may be potentially used for enhanced treatment of breast cancer.
合成 HSA-RB-DOX 纳米粒，测定其性质并初步评价其抗癌作用。将阿霉素（DOX）和玫瑰红（RB）共同包载于白蛋白载体中。通过 RB 诱导白蛋白自组装制备 HSA-RB-DOX 纳米粒。测定其性质并在 MCF-7 细胞和荷瘤小鼠中检测其抗癌作用。平均粒径为 42nm 的 HSA-RB-DOX 纳米粒在不同介质中稳定，表现出控制释放特性。它能很好地被 MCF-7 细胞摄取，并通过诱导活性氧（ROS）的产生抑制 MCF-7 细胞增殖。它在血液中的浓度保持在较高水平长达 12 小时，在肿瘤组织中的积累也更多。在荷瘤小鼠中，HSA-RB-DOX 纳米粒抑制肿瘤生长，甚至使肿瘤体积从 100 减少到 50mm，对体重几乎没有影响。HSA-RB-DOX 纳米粒可能具有增强乳腺癌治疗的潜力。
