Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, and Beijing Key Laboratory of Digital Stomatology, Beijing, China.
Institute of Systems Biomedicine and Department of Ophthalmology, School of Basic Medical Sciences, Peking University Third Hospital, Beijing, China.
Stem Cells Dev. 2019 Nov 15;28(22):1514-1526. doi: 10.1089/scd.2019.0158. Epub 2019 Nov 4.
Retinitis pigmentosa (RP) is a hereditary disease characterized by degeneration and the loss of photoreceptors. Stem cell-based therapy has emerged as a promising strategy for treating RP. Stem cells from exfoliated deciduous teeth (SHEDs), a type of mesenchymal stem cell from human exfoliated deciduous teeth, have the potential to differentiate into photoreceptor-like cells under specific induction in vitro. It has been confirmed that through paracrine secreta, SHEDs exert neurotrophic, angiogenic, immunoregulatory, and antiapoptotic functions in injured tissues. This study was designed to determine whether retinal-differentiated SHEDs and the conditioned medium derived from SHEDs (SHEDs-CM) have therapeutic effects in a mouse model of RP. The results showed that both SHEDs and SHEDs-CM improved electroretinogram responses, ameliorated photoreceptor degeneration, and maintained the structure of the outer segments of photoreceptors. The therapeutic effects were related to antiapoptotic activity of SHEDs and SHEDs-CM. Thus, SHEDs may be a promising stem cell source for treating retinal degeneration.
色素性视网膜炎（RP）是一种遗传性疾病，其特征是感光细胞退化和丧失。基于干细胞的治疗方法已成为治疗 RP 的一种有前途的策略。脱落乳牙来源的干细胞（SHED）是一种源自人类脱落乳牙的间充质干细胞，具有在体外特定诱导下分化为光感受器样细胞的潜力。已经证实，SHED 通过旁分泌分泌，在受损组织中发挥神经营养、血管生成、免疫调节和抗细胞凋亡作用。本研究旨在确定视网膜分化的 SHED 和 SHED 来源的条件培养基（SHED-CM）在 RP 小鼠模型中是否具有治疗作用。结果表明，SHED 和 SHED-CM 均改善了视网膜电图反应，改善了光感受器变性，并维持了光感受器外节的结构。治疗效果与 SHED 和 SHED-CM 的抗细胞凋亡活性有关。因此，SHED 可能是治疗视网膜变性的一种有前途的干细胞来源。
