State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
Theranostics. 2019 Apr 13;9(9):2694-2711. doi: 10.7150/thno.31801. eCollection 2019.
A stem cell-mediated bioengineered tooth root (bio-root) has proven to be a prospective tool for the treatment of tooth loss. As shown in our previous studies, dental follicle cells (DFCs) are suitable seeding cells for the construction of bio-roots. However, the DFCs which can only be obtained from unerupted tooth germ are restricted. Stem cells from human exfoliated deciduous teeth (SHEDs), which are harvested much more easily through a minimally invasive procedure, may be used as an alternative seeding cell. In this case, we compared the odontogenic characteristics of DFCs and SHEDs in bio-root regeneration. : The biological characteristics of SHEDs and DFCs were determined . The cells were then induced to secrete abundant extracellular matrix (ECM) and form macroscopic cell sheets. We combined the cell sheets with treated dentin matrix (TDM) for subcutaneous transplantation into nude mice and orthotopic jaw bone implantation in Sprague-Dawley rats to further verify their regenerative potential. : DFCs exhibited a higher proliferation rate and stronger osteogenesis and adipogenesis capacities, while SHEDs displayed increased migration ability and excellent neurogenic potential. Both dental follicle cell sheets (DFCSs) and sheets of stem cells from human exfoliated deciduous teeth (SHEDSs) expressed not only ECM proteins but also osteogenic and odontogenic proteins. Importantly, similar to DFCSs/TDM, SHEDSs/TDM also successfully achieved the regeneration of the periodontal tissues, which consist of periodontal ligament fibers, blood vessels and new born alveolar bone. : Both SHEDs and DFCs possessed a similar odontogenic differentiation capacity and SHEDs were regarded as a prospective seeding cell for use in bio-root regeneration in the future.
一种基于干细胞的生物工程牙根（生物根）已被证明是治疗牙齿缺失的有前途的工具。如我们之前的研究所示，牙囊细胞（DFC）是构建生物根的合适种子细胞。然而，只能从未萌出牙胚中获得 DFC，受到限制。从人类脱落乳牙（SHED）中获取的干细胞，通过微创程序更容易获得，可作为替代种子细胞。在这种情况下，我们比较了生物根再生中 DFC 和 SHED 的成牙特性。我们确定了 SHED 和 DFC 的生物学特性。然后将细胞诱导分泌大量细胞外基质（ECM）并形成宏观细胞片。我们将细胞片与处理过的牙本质基质（TDM）结合，进行皮下移植到裸鼠和 Sprague-Dawley 大鼠的原位颌骨植入，以进一步验证它们的再生潜力。DFC 表现出更高的增殖率和更强的成骨和成脂能力，而 SHED 则表现出更强的迁移能力和优异的神经发生潜力。牙囊细胞片（DFCSs）和人脱落乳牙干细胞片（SHEDSs）不仅表达 ECM 蛋白，而且表达成骨和成牙蛋白。重要的是，类似于 DFCSs/TDM，SHEDSs/TDM 也成功实现了牙周组织的再生，包括牙周韧带纤维、血管和新生牙槽骨。SHED 和 DFC 均具有相似的成牙分化能力，并且 SHED 被认为是未来生物根再生的有前途的种子细胞。
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