Abla Merhaba, Zha Xi, Wang Ying, Yang Wang Xiao, Gao Fei, Zhou Yijun, Feng Jinchao
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, People's Republic of China.
J Genet. 2019 Sep;98.
The chloroplast genome sequence of , a desert shrub, was sequenced using high-throughput sequencing technology and analysed phylogenetically in the present study. The chloroplast genome is 159,414 bp in length, including a large single copy region of 87,924 bp and small single copy region of 18,318 bp, and a pair of inverted repeat regions of 26,586 bp. The chloroplast genome contains 110 unique genes, including 77 protein-coding genes, four ribosomal RNA genes, and 29 tRNA genes. Most of these genes are present as a single copy and in two or more copies 19 genes occurred. Seventeen genes have one intron, and and genes contain two introns. A total of 81 simple sequence repeats (SSRs) were identified, most of them were found to be mononucleotide repeats composed of A/T. In addition to SSRs, 66 repeats were identified, including 41 tandem repeats, 10 palindromic repeats, and 15 forward repeats. The phylogenetic analysis based on 54 protein-coding genes demonstrated a close relationship between and other plant species in Sapindales. The complete chloroplast genome sequence of will provide important data for further study of taxonomy and systematics of the genus Nitraria.
本研究利用高通量测序技术对一种沙漠灌木的叶绿体基因组序列进行了测序,并进行了系统发育分析。该叶绿体基因组长度为159,414 bp,包括一个87,924 bp的大单拷贝区域、一个18,318 bp的小单拷贝区域以及一对26,586 bp的反向重复区域。叶绿体基因组包含110个独特基因,其中包括77个蛋白质编码基因、4个核糖体RNA基因和29个tRNA基因。这些基因大多以单拷贝形式存在,19个基因以两个或更多拷贝形式出现。17个基因有一个内含子, 和 基因含有两个内含子。共鉴定出81个简单序列重复(SSR),其中大部分是由A/T组成的单核苷酸重复。除SSR外,还鉴定出66个重复序列,包括41个串联重复、10个回文重复和15个正向重复。基于54个蛋白质编码基因的系统发育分析表明, 与无患子目其他植物物种关系密切。 的完整叶绿体基因组序列将为进一步研究白刺属的分类学和系统学提供重要数据。