野生黄瓜（黄瓜变种哈氏黄瓜）的完整叶绿体基因组序列。
The complete chloroplast genome sequence of wild cucumber (Cucumis sativus var. Hardwickii).
作者信息
Gao Ju, Liu Bang, Jiang Honghai
机构信息
a Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming , China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4524-4525. doi: 10.3109/19401736.2015.1101545. Epub 2015 Nov 5.
The complete chloroplast genome sequence of wild cucumber (Cucumis sativus var. hardwickii) was generated by de novo assembly with low-coverage whole-genome sequence data. The 155 277 bp genome containing a pair of inverted repeats (IRs) of 25 198 bp separated by a large single-copy region of 86 618 bp and a small single-copy region of 18 263 bp. The chloroplast genome contains 130 known genes, including 89 protein-coding genes, eight rRNA genes (four kinds), and 37 tRNA genes (30 kinds). Eighteen genes are duplicated in the inverted repeat regions, 16 genes contain one intron, two genes, and one ycf contain two introns. Phylogenomic analysis showed that C. sativus var. Hardwickii is closely related to C. sativus and C. hystrix.
通过对低覆盖度全基因组序列数据进行从头组装，获得了野生黄瓜（Cucumis sativus var. hardwickii）的完整叶绿体基因组序列。该基因组大小为155277 bp，包含一对25198 bp的反向重复序列（IRs），被一个86618 bp的大单拷贝区域和一个18263 bp的小单拷贝区域隔开。叶绿体基因组包含130个已知基因，其中包括89个蛋白质编码基因、8个rRNA基因（4种）和37个tRNA基因（30种）。18个基因在反向重复区域中重复，16个基因含有一个内含子，两个基因和一个ycf含有两个内含子。系统发育基因组学分析表明，C. sativus var. Hardwickii与C. sativus和C. hystrix密切相关。
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