Subtyping Lung Cancer Using DNA Methylation in Liquid Biopsies.

BACKGROUND

Lung cancer (LCa) is the most frequently diagnosed and lethal cancer worldwide. Histopathological subtyping, which has important therapeutic and prognostic implications, requires material collection through invasive procedures, which might be insufficient to enable definitive diagnosis. Aberrant DNA methylation is an early event in carcinogenesis, detectable in circulating cell-free DNA (ccfDNA). Herein, we aimed to assess methylation of selected genes in ccfDNA from LCa patients and determine its accuracy for tumor subtyping.

METHODS

Methylation levels of , , and were assessed in three independent study groups (study group #1: 152 tissue samples; study group #2: 129 plasma samples; study group #3: 28 benign lesions of lung) using quantitative methylation-specific PCR. Associations between gene promoter methylation levels and LCa subtypes were evaluated using non-parametric tests. Receiver operating characteristic (ROC) curve analysis was performed.

RESULTS

In study group #2, and displayed higher methylation levels in small-cell lung cancer (SCLC) than in non-small-cell lung cancer (NSCLC). displayed high sensitivity (63.8%), whereas disclosed high specificity (96.2%) for SCLC detection in ccfDNA. Furthermore, methylation levels showed to be higher in squamous cell carcinoma in comparison with adenocarcinoma in study group #1.

CONCLUSIONS

Methylation level assessments in ccfDNA may provide a minimally invasive procedure for LCa subtyping, complementing standard diagnostic procedures.