Laboratorio de Genómica Humana, Facultad de Medicina, Universidad Veracruzana, Médicos y Odontólogos S/N, Col. Unidad del Bosque, Xalapa C.P. 91010, Veracruz, Mexico.
LADISER, Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba 94340, Veracruz, Mexico.
Biomolecules. 2019 Sep 12;9(9):484. doi: 10.3390/biom9090484.
, which causes Chagas disease, is a significant health threat in many countries and affects millions of people. Given the magnitude of this disease, a broader understanding of trypanocidal mechanisms is needed to prevent and treat infection. Natural endoperoxides, such as ergosterol peroxide, have been shown to be toxic to parasites without causing harm to human cells or tissues. Although prior studies have demonstrated the trypanocidal activity of ergosterol peroxide, the cellular and molecular mechanisms remain unknown. The results of this study indicate that a free-radical reaction occurs in following ergosterol peroxide exposure, leading to cell death. Using a combination of biochemical, microscopic and in silico experimental approaches, we have identified, for the first time, the cellular and molecular cytotoxic mechanism of an ergosterol peroxide obtained from (Jacq) P. Kumm. f. sp. Florida.
克氏锥虫，引起恰加斯病，在许多国家是一个重大的健康威胁，影响了数百万人。鉴于这种疾病的严重性，需要更广泛地了解杀锥虫机制，以预防和治疗感染。天然过氧化物，如麦角甾醇过氧化物，已被证明对寄生虫有毒，而不会对人体细胞或组织造成伤害。尽管先前的研究已经证明了麦角甾醇过氧化物的杀锥虫活性，但细胞和分子机制仍不清楚。本研究结果表明，在麦角甾醇过氧化物暴露后，会发生自由基反应，导致细胞死亡。本研究首次使用生化、显微镜和计算机实验方法相结合，鉴定了来自佛罗里达亚种（Jacq）P. Kumm. f. 的麦角甾醇过氧化物的细胞和分子细胞毒性机制。
