Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Sci Adv. 2019 Sep 13;5(9):eaaw0672. doi: 10.1126/sciadv.aaw0672. eCollection 2019 Sep.
NIR-II fluorescence imaging greatly reduces scattering coefficients for nearly all tissue types at long wavelengths, benefiting deep tissue imaging. However, most of the NIR-II fluorophores suffer from low quantum yields and/or short circulation time that limit the quality of NIR-II imaging. Here, we engineered a supramolecular assembly of protein complex with lodged cyanine dyes to produce a brilliant NIR-II fluorophore, providing a NIR-II quantum yield of 21.2% with prolonged circulation time. Computational modeling revealed the mechanism for fluorescence enhancement and identified key parameters governing albumin complex for NIR-II fluorophores. Our complex afforded high-resolution microvessel imaging, with a 3-hour imaging window compared to 2 min for free dye alone. Furthermore, the complexation strategy was applied to an antibody-derived assembly, offering high-contrast tumor imaging without affecting the targeting ability of the antibody. This study provides a facile strategy for producing high-performance NIR-II fluorophores by chaperoning cyanine dyes with functional proteins.
近红外二区（NIR-II）荧光成像是一种在长波长下能极大降低几乎所有组织散射系数的技术，有利于深层组织成像。然而，大多数近红外二区荧光染料的量子产率低和/或循环时间短，限制了近红外二区成像的质量。在这里，我们通过将菁染料嵌入蛋白质复合物来构建超分子组装体，从而产生了一种明亮的近红外二区荧光染料，其量子产率为 21.2%，循环时间延长。计算模型揭示了荧光增强的机制，并确定了控制白蛋白复合物作为近红外二区荧光染料的关键参数。我们的复合物实现了高分辨率微血管成像，与单独使用游离染料的 2 分钟相比，具有 3 小时的成像窗口。此外，该组装策略还应用于抗体衍生的组装体，在不影响抗体靶向能力的情况下提供高对比度的肿瘤成像。这项研究通过用功能蛋白包裹菁染料为制备高性能近红外二区荧光染料提供了一种简便的策略。
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