Institut Agronomique et Vétérinaire Hassan II, Unité Parasitologie et Maladies Parasitaires, 10100, Rabat, Morocco.
ASTRE, Univ Montpellier, Cirad, INRA, Montpellier, France.
Parasit Vectors. 2019 Sep 24;12(1):459. doi: 10.1186/s13071-019-3720-4.
Investigations of Culicoides fauna, including inventories, were carried out in Morocco at different periods after the country had faced major bluetongue and African horse sickness outbreaks. However, no comprehensive reference publication has provided a clear overview of the Culicoides species diversity. This study reviewed available data on Culicoides biting midge species in Morocco from 1968 to 2015 (published and grey literature in French and English) in order to revise the current checklist, in light of state of the art taxonomic and systematic knowledge, and confirmed the checklist with morphological and molecular identifications of specimens collected from the region of Rabat.
Literature related to Culicoides collections in Morocco was collated. Authors were contacted to obtain raw data and additional information for the collections. Fresh Culicoides material was collected and examined from two sites around Rabat, the capital of Morocco. Each collected individual was examined and morphologically identified, if possible, to the species level. In addition, molecular identification was performed to separate closely related species, to confirm difficult morphological identifications and to confirm new species records.
A total of 6121 individuals of Culicoides spp. were collected and analyzed and at least 17 species were identified: C. cataneii/C. gejgelensis, C. circumscriptus, C. fagineus, C. festivipennis, C. imicola, C. jumineri, C. kingi, C. longipennis, C. montanus, C. newsteadi, C. obsoletus, C. paolae, C. parotti, C. puncticollis, C. sahariensis, C. scoticus and C. subfagineus. Seven species were confirmed using phylogenetic analyses. Two new species records for Morocco are reported: C. paolae and C. subfagineus.
The Moroccan fauna of Culicoides now includes 54 valid species. Further work would certainly increase this total, as one of the clades we identified was not affiliated to any described and valid species.
在摩洛哥，该国经历了重大的蓝舌病和非洲马瘟疫情爆发后，不同时期都开展了库蠓动物区系的调查，包括清查工作。然而，没有任何综合参考出版物能清晰概述库蠓物种的多样性。本研究审查了 1968 年至 2015 年期间在摩洛哥有关库蠓吸血蠓种类的现有数据（用法语和英语出版和灰色文献），以便根据最新的分类和系统知识修订现行清单，并通过对从拉巴特地区收集的标本进行形态和分子鉴定来确认清单。
整理了与摩洛哥库蠓收集相关的文献。联系作者以获取有关收集的原始数据和其他信息。从摩洛哥首都拉巴特附近的两个地点采集新鲜的库蠓材料并进行检查。对每一个采集的个体进行检查，如果可能的话，尽可能鉴定到种的水平。此外，还进行了分子鉴定，以区分密切相关的物种，确认难以鉴定的形态和确认新的物种记录。
共收集和分析了 6121 只库蠓属个体，至少鉴定出 17 种：C. cataneii/C. gejgelensis、C. circumscriptus、C. fagineus、C. festivipennis、C. imicola、C. jumineri、C. kingi、C. longipennis、C. montanus、C. newsteadi、C. obsoletus、C. paolae、C. parotti、C. puncticollis、C. sahariensis、C. scoticus 和 C. subfagineus。通过系统发育分析确认了 7 个物种。报告了摩洛哥的两个新物种记录：C. paolae 和 C. subfagineus。
摩洛哥的库蠓动物区系现在包括 54 个有效物种。进一步的工作肯定会增加这个总数，因为我们鉴定的一个分支与任何描述的有效物种都没有关联。
