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在无菌培养条件下以及在植物内病原菌-油棕(Elaeis guineensis Jacq.)相互作用情况下的灵芝RNA测序数据。

RNA-seq data of Ganoderma boninense at axenic culture condition and under in planta pathogen-oil palm (Elaeis guineensis Jacq.) interaction.

作者信息

Wong Mui Yun, Govender Nisha T, Ong Chia Sui

机构信息

Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Institute of Plantation Studies, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

出版信息

BMC Res Notes. 2019 Sep 24;12(1):631. doi: 10.1186/s13104-019-4652-y.

DOI:10.1186/s13104-019-4652-y
PMID:31551084
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6760109/
Abstract

OBJECTIVE

Basal stem rot disease causes severe economic losses to oil palm production in South-east Asia and little is known on the pathogenicity of the pathogen, the basidiomyceteous Ganoderma boninense. Our data presented here aims to identify both the house-keeping and pathogenicity genes of G. boninense using Illumina sequencing reads.

DESCRIPTION

The hemibiotroph G. boninense establishes via root contact during early stage of colonization and subsequently kills the host tissue as the disease progresses. Information on the pathogenicity factors/genes that causes BSR remain poorly understood. In addition, the molecular expressions corresponding to G. boninense growth and pathogenicity are not reported. Here, six transcriptome datasets of G. boninense from two contrasting conditions (three biological replicates per condition) are presented. The first datasets, collected from a 7-day-old axenic condition provide an insight onto genes responsible for sustenance, growth and development of G. boninense while datasets of the infecting G. boninense collected from oil palm-G. boninense pathosystem (in planta condition) at 1 month post-inoculation offer a comprehensive avenue to understand G. boninense pathogenesis and infection especially in regard to molecular mechanisms and pathways. Raw sequences deposited in Sequence Read Archive (SRA) are available at NCBI SRA portal with PRJNA514399, bioproject ID.

摘要

目的

基部干腐病给东南亚的油棕生产造成了严重的经济损失,而对于病原菌——担子菌纲的邦氏灵芝的致病性却知之甚少。我们在此展示的数据旨在利用Illumina测序读数鉴定邦氏灵芝的持家基因和致病基因。

描述

半活体营养型的邦氏灵芝在定殖早期通过根部接触定殖,随后随着病情发展杀死宿主组织。关于导致基部干腐病的致病因子/基因的信息仍然了解不足。此外,尚未报道与邦氏灵芝生长和致病性相对应的分子表达情况。在此,展示了来自两种不同条件(每种条件三个生物学重复)的六个邦氏灵芝转录组数据集。第一个数据集是从7日龄无菌条件下收集的,可深入了解负责邦氏灵芝存活、生长和发育的基因,而从接种后1个月的油棕 - 邦氏灵芝病理系统(植物体内条件)收集的感染邦氏灵芝的数据集提供了一个全面的途径来了解邦氏灵芝的发病机制和感染,特别是在分子机制和途径方面。保存在序列读取存档(SRA)中的原始序列可在NCBI SRA门户上获取,生物项目ID为PRJNA514399。

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本文引用的文献

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Draft Genome Sequence of the Phytopathogenic Fungus Ganoderma boninense, the Causal Agent of Basal Stem Rot Disease on Oil Palm.油棕基部干腐病病原菌——邦那灵芝的基因组序列草图
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Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense.从致病的博宁灵芝子实体中获得担孢子和原生质体再生
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