Martins Maíra Pompeu, Martinez-Rossi Nilce M, Sanches Pablo R, Gomes Eriston Vieira, Bertolini Maria Célia, Pedersoli Wellington R, Silva Roberto Nascimento, Rossi Antonio
Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Department of Biofunctional, Morgana Potrich College, Mineiros, Brazil.
Front Microbiol. 2019 Sep 4;10:2076. doi: 10.3389/fmicb.2019.02076. eCollection 2019.
The zinc finger transcription factor PAC-3/RIM101/PacC has a defined role in the secretion of enzymes and proteins in response to ambient pH, and also contributes to the virulence of species. Herein we evaluated the role of PAC-3 in the regulation of genes, in a model that examined the plant-fungi interactions. is a model fungal species capable of exhibiting dynamic responses to its environment by employing endophytic or phytopathogenic behavior according to a given circumstance. Since plant growth and productivity are highly affected by pH and phosphorus (P) acquisition, we sought to verify the impact that induction of a Δ mutation would have under limited and sufficient Pi availability, while ensuring that the targeted physiological adjustments mimicked ambient pH and nutritional conditions required for efficient fungal growth and development. Our results suggest direct regulatory functions for PAC-3 in cell wall biosynthesis, homeostasis, oxidation-reduction processes, hydrolase activity, transmembrane transport, and modulation of genes associated with fungal virulence. Pi-dependent modulation was observed mainly in genes encoding for transporter proteins or related to cell wall development, thereby advancing the current understanding regarding colonization and adaptation processes in response to challenging environments. We have also provided comprehensive evidence that suggests a role for PAC-3 as a global regulator in plant pathogenic fungi, thus presenting results that have the potential to be applied to various types of microbes, with diverse survival mechanisms.
锌指转录因子PAC-3/RIM101/PacC在响应环境pH值时对酶和蛋白质的分泌具有明确作用，并且也有助于物种的毒力。在此，我们在一个研究植物与真菌相互作用的模型中评估了PAC-3在基因调控中的作用。是一种模式真菌物种，能够根据特定情况通过采用内生或植物致病行为对其环境表现出动态反应。由于植物生长和生产力受到pH值和磷（P）获取的高度影响，我们试图验证在有限和充足的磷可用性条件下，Δ突变的诱导所产生的影响，同时确保目标生理调节模拟有效真菌生长和发育所需的环境pH值和营养条件。我们的结果表明PAC-3在细胞壁生物合成、稳态、氧化还原过程、水解酶活性、跨膜运输以及与真菌毒力相关基因的调节中具有直接调控功能。主要在编码转运蛋白或与细胞壁发育相关的基因中观察到磷依赖性调节，从而推进了目前对响应挑战性环境的定殖和适应过程的理解。我们还提供了全面的证据，表明PAC-3在植物致病真菌中作为全局调节因子的作用，从而呈现出有可能应用于具有不同生存机制的各种类型微生物的结果。
