Zhao Hua, Harter Glenn A, Martin Caden J
Department of Chemistry and Biochemistry, University of Northern Colorado, Greeley, Colorado 80639, United States.
ACS Omega. 2019 Sep 4;4(12):15234-15239. doi: 10.1021/acsomega.9b02118. eCollection 2019 Sep 17.
By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and -alcohol groups (e.g., [CH(OCHCH) -Im--BuOH][TfN]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from , which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][TfN]) and up to 40-100% higher than diisopropyl ether and -butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than -butanol.
通过模拟水结构来提高酶活性，我们设计了同时带有醚基和醇基官能团的咪唑鎓（Im）基离子液体（ILs）（例如，[CH(OCHCH) -Im--BuOH][TfN]）。这种独特的“类水”结构组合使得来自的固定化脂肪酶B具有非常高的酯交换（合成）活性，比未官能化的“经典”离子液体（如[BMIM][TfN]）高出2至4倍，比二异丙醚和丁醇高出40%至100%。荧光发射光谱证实了在这些定制的离子溶剂中蛋白质的总体结构得以保留。此外，官能化离子液体表现出高热稳定性，与二异丙醚相当，但远高于丁醇。
