Mandelstam J, Errington J
Department of Biochemistry, University of Oxford, South Parks, UK.
Microbiol Sci. 1987 Aug;4(8):238-44.
Sporulation in Bacillus subtilis is a simple form of differentiation that involves the formation of a two-cell organism; mother cell and prospective spore. It is regulated by at least 50 operons, some mono- and some polycistronic. These are expressed in a dependent sequence which branches at an early stage so that there are independent lines of expression in both cells; further ramifications then occur in both cell compartments. Three DNA-binding proteins and two sigma factors have so far been identified as probable regulators of the dependent sequence. Most of the operons of the sequence are expressed in the first four hours and the last two stages occur mainly by self-assembly of proteins.
枯草芽孢杆菌中的孢子形成是一种简单的分化形式，涉及形成一种双细胞生物体，即母细胞和预期的孢子。它至少由50个操纵子调控，有些是单顺反子的，有些是多顺反子的。这些操纵子按依赖序列表达，该序列在早期分支，因此两个细胞中有独立的表达途径；随后在两个细胞区室中都会进一步分支。到目前为止，已鉴定出三种DNA结合蛋白和两种σ因子可能是该依赖序列的调节因子。该序列的大多数操纵子在最初的四个小时内表达，最后两个阶段主要通过蛋白质的自组装发生。
