枯草芽孢杆菌内源性β-半乳糖苷酶及其调节因子编码基因突变的分离与鉴定
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
作者信息
Errington J, Vogt C H
机构信息
Department of Biochemistry, University of Oxford, United Kingdom.
出版信息
J Bacteriol. 1990 Jan;172(1):488-90. doi: 10.1128/jb.172.1.488-490.1990.
Abstract
We have isolated mutations that appear to inactivate the gene (lacA) encoding an endogenous beta-galactosidase activity in Bacillus subtilis and in a closely linked negative regulatory element (lacR). Both genes map to the hisA-thrA region. The lacA mutations may help to avoid some of the problems arising from the use of the Escherichia coli lacZ gene as a reporter gene in B. subtilis.
摘要
我们已经分离出一些突变，这些突变似乎使编码枯草芽孢杆菌内源性β-半乳糖苷酶活性的基因（lacA）以及一个紧密连锁的负调控元件（lacR）失活。这两个基因都定位于hisA-thrA区域。lacA突变可能有助于避免在枯草芽孢杆菌中使用大肠杆菌lacZ基因作为报告基因所产生的一些问题。
相似文献
1
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol. 1990 Jan;172(1):488-90. doi: 10.1128/jb.172.1.488-490.1990.
2
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol. 1997 Sep;179(17):5636-8. doi: 10.1128/jb.179.17.5636-5638.1997.
3
Use of lacZ gene fusions to determine the dependence pattern of the sporulation gene spoIID in spo mutants of Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2987-94. doi: 10.1099/00221287-132-11-2987.
4
Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis.
Gene. 1986;43(1-2):85-94. doi: 10.1016/0378-1119(86)90011-9.
5
[Cloning of the Streptococcus thermophilus beta-galactosidase gene and its expression in Escherichia coli and Bacillus subtilis cells].
Mol Gen Mikrobiol Virusol. 1990 Apr(4):10-4.
6
Use of a lacZ gene fusion to determine the dependence pattern and the spore compartment expression of sporulation operon spoVA in spo mutants of Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2977-85. doi: 10.1099/00221287-132-11-2977.
7
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2967-76. doi: 10.1099/00221287-132-11-2967.
8
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons.
J Gen Microbiol. 1986 Nov;132(11):2995-3003. doi: 10.1099/00221287-132-11-2995.
9
Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01.
BMC Biochem. 2016 Jul 8;17(1):15. doi: 10.1186/s12858-016-0070-0.
10
A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2953-66. doi: 10.1099/00221287-132-11-2953.
引用本文的文献
1
Characterization of the regulation of a plant polysaccharide utilization operon and its role in biofilm formation in Bacillus subtilis.
PLoS One. 2017 Jun 15;12(6):e0179761. doi: 10.1371/journal.pone.0179761. eCollection 2017.
2
Linearmycins Activate a Two-Component Signaling System Involved in Bacterial Competition and Biofilm Morphology.
J Bacteriol. 2017 Aug 22;199(18). doi: 10.1128/JB.00186-17. Print 2017 Sep 15.
3
Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.
J Bacteriol. 2016 Sep 22;198(20):2887-96. doi: 10.1128/JB.00468-16. Print 2016 Oct 15.
4
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol. 2006 Dec;72(12):7730-8. doi: 10.1128/AEM.01306-06. Epub 2006 Oct 20.
5
The Bacillus subtilis galE gene is essential in the presence of glucose and galactose.
J Bacteriol. 1998 Apr;180(8):2265-70. doi: 10.1128/JB.180.8.2265-2270.1998.
6
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol. 1997 Sep;179(17):5636-8. doi: 10.1128/jb.179.17.5636-5638.1997.
7
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8841-5. doi: 10.1073/pnas.93.17.8841.
8
Role of CodY in regulation of the Bacillus subtilis hut operon.
J Bacteriol. 1996 Jul;178(13):3779-84. doi: 10.1128/jb.178.13.3779-3784.1996.
9
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
J Bacteriol. 1996 Apr;178(8):2343-50. doi: 10.1128/jb.178.8.2343-2350.1996.
10
Identification of a Bacillus thuringiensis gene that positively regulates transcription of the phosphatidylinositol-specific phospholipase C gene at the onset of the stationary phase.
J Bacteriol. 1996 May;178(10):2749-56. doi: 10.1128/jb.178.10.2749-2756.1996.
本文引用的文献
1
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
2
Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis.
J Gen Microbiol. 1983 Jun;129(6):1945-58. doi: 10.1099/00221287-129-6-1945.
3
Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.
Cell. 1983 Nov;35(1):275-83. doi: 10.1016/0092-8674(83)90230-1.
4
Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis.
J Bacteriol. 1966 May;91(5):1795-803. doi: 10.1128/jb.91.5.1795-1803.1966.
5
Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants.
J Gen Microbiol. 1972 Jun;71(1):17-27. doi: 10.1099/00221287-71-1-17.
6
Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance.
Biochem J. 1969 Jun;113(1):29-37. doi: 10.1042/bj1130029.
7
Dependent sequences of gene expression controlling spore formation in Bacillus subtilis.
Microbiol Sci. 1987 Aug;4(8):238-44.
8
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons.
J Gen Microbiol. 1986 Nov;132(11):2995-3003. doi: 10.1099/00221287-132-11-2995.
9
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2967-76. doi: 10.1099/00221287-132-11-2967.
10
A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis.
J Gen Microbiol. 1986 Nov;132(11):2953-66. doi: 10.1099/00221287-132-11-2953.
Zotero 插件