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枯草芽孢杆菌内源性β-半乳糖苷酶及其调节因子编码基因突变的分离与鉴定

Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.

作者信息

Errington J, Vogt C H

机构信息

Department of Biochemistry, University of Oxford, United Kingdom.

出版信息

J Bacteriol. 1990 Jan;172(1):488-90. doi: 10.1128/jb.172.1.488-490.1990.

DOI:10.1128/jb.172.1.488-490.1990
PMID:2104611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208459/
Abstract

We have isolated mutations that appear to inactivate the gene (lacA) encoding an endogenous beta-galactosidase activity in Bacillus subtilis and in a closely linked negative regulatory element (lacR). Both genes map to the hisA-thrA region. The lacA mutations may help to avoid some of the problems arising from the use of the Escherichia coli lacZ gene as a reporter gene in B. subtilis.

摘要

我们已经分离出一些突变，这些突变似乎使编码枯草芽孢杆菌内源性β-半乳糖苷酶活性的基因（lacA）以及一个紧密连锁的负调控元件（lacR）失活。这两个基因都定位于hisA-thrA区域。lacA突变可能有助于避免在枯草芽孢杆菌中使用大肠杆菌lacZ基因作为报告基因所产生的一些问题。

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REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
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Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons.利用lacZ基因融合来确定枯草芽孢杆菌spo突变体中孢子形成操纵子spoIIIC的依赖性模式：孢子形成操纵子表达的分支途径
J Gen Microbiol. 1986 Nov;132(11):2995-3003. doi: 10.1099/00221287-132-11-2995.
9
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis.利用lacZ基因融合来确定枯草芽孢杆菌spo突变体中芽孢形成操纵子spoIIA的依赖模式。
J Gen Microbiol. 1986 Nov;132(11):2967-76. doi: 10.1099/00221287-132-11-2967.
10
A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis.一种将大肠杆菌lacZ基因与枯草芽孢杆菌染色体基因融合的通用方法。
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