Varvara Palla Viktoria, Karaolanis Georgios, Valavanis Christos, Stanc Gabriela, Tzaida Olympia, Trihia Helen, Patapis Paul, Dimitroulis Dimitrios, Perrea Despoina
Department of Obstetrics and Gynecology, Diakonie-Klinikum Schwäbisch Hall, Schwäbisch Hall, Germany.
1st Department of Surgery, Vascular Unit, Laiko General Hospital, Medical School of Athens, Athens, Greece.
Tumour Biol. 2019 Sep;41(9):1010428319878536. doi: 10.1177/1010428319878536.
Histone H2AX undergoes phosphorylation as an answer to DNA double-strand breaks, which in turn are part of the oncogenic procedure. The detection of gamma-H2AX can potentially serve as a biomarker for transformation of normal tissue to premalignant and consequently to malignant tissues. The aim of this study was to evaluate the clinical significance of gamma-H2AX expression in breast cancer. Gamma-H2AX expression in tissues from 110 breast cancer patients was analyzed by immunohistochemistry and correlated with clinicopathological variables. Greater tumor size, higher grade, and the number of affected lymph nodes are significantly associated with greater values of gamma-H2AX. In addition, gamma-H2AX differs significantly among patients' International Federation of Gynecology and Obstetrics stage. Higher values of estrogen receptor and progesterone receptor are significantly associated with lower gamma-H2AX values. In conclusion, a positive association between gamma-H2AX expression and infaust histopathological parameters was observed.
组蛋白H2AX会发生磷酸化以应对DNA双链断裂，而DNA双链断裂又是致癌过程的一部分。检测γ-H2AX有可能作为正常组织转变为癌前组织进而转变为恶性组织的生物标志物。本研究的目的是评估γ-H2AX表达在乳腺癌中的临床意义。通过免疫组织化学分析了110例乳腺癌患者组织中的γ-H2AX表达，并将其与临床病理变量相关联。更大的肿瘤大小、更高的分级以及受累淋巴结数量与γ-H2AX的更高值显著相关。此外，γ-H2AX在患者的国际妇产科联盟分期中存在显著差异。雌激素受体和孕激素受体的较高值与较低的γ-H2AX值显著相关。总之，观察到γ-H2AX表达与不良组织病理学参数之间存在正相关。
